ArticleMolecular and cellular biology2005
A novel role for the mitotic spindle during DNA segregation in yeast: promoting 2 microm plasmid-cohesin association.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- pSB3, a member of the 2-micron family of plasmids, replicates and is efficiently partitioned in multiple budding yeast species.G3 (Bethesda, Md.) · 2026Article
- The selfish yeast plasmid exploits a SWI/SNF-type chromatin remodeling complex for hitchhiking on chromosomes and ensuring high-fidelity propagation.PLoS genetics · 2023Article
- The yeast 2-micron plasmid Rep2 protein has Rep1-independent partitioning function.Nucleic acids research · 2022Article
- The selfish yeast plasmid utilizes the condensin complex and condensed chromatin for faithful partitioning.PLoS genetics · 2021Article
- Insights into the DNA sequence elements required for partitioning and copy number control of the yeast 2-micron plasmid.Current genetics · 2019Review
- DNA sequence elements required for partitioning competence of the Saccharomyces cerevisiae 2-micron plasmid STB locus.Nucleic acids research · 2019Article
- The 2 micron plasmid: a selfish genetic element with an optimized survival strategy within Saccharomyces cerevisiae.Current genetics · 2018Review
- The 2-μm plasmid encoded protein Raf1 regulates both stability and copy number of the plasmid by blocking the formation of the Rep1-Rep2 repressor complex.Nucleic acids research · 2017Article
- Replication-dependent and independent mechanisms for the chromosome-coupled persistence of a selfish genome.Nucleic acids research · 2016Article
- The partitioning and copy number control systems of the selfish yeast plasmid: an optimized molecular design for stable persistence in host cells.Microbiology spectrum · 2014Review
- A selfish DNA element engages a meiosis-specific motor and telomeres for germ-line propagation.The Journal of cell biology · 2014Article
- Co-segregation of yeast plasmid sisters under monopolin-directed mitosis suggests association of plasmid sisters with sister chromatids.Nucleic acids research · 2013Article
- Temporal sequence and cell cycle cues in the assembly of host factors at the yeast 2 micron plasmid partitioning locus.Nucleic acids research · 2013Article
- Article
- CENP-A exceeds microtubule attachment sites in centromere clusters of both budding and fission yeast.The Journal of cell biology · 2011Article
- Histone H3-variant Cse4-induced positive DNA supercoiling in the yeast plasmid has implications for a plasmid origin of a chromosome centromere.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Cse4 (CenH3) association with the Saccharomyces cerevisiae plasmid partitioning locus in its native and chromosomally integrated states: implications in centromere evolution.Molecular and cellular biology · 2011Article
- Yeast cohesin complex embraces 2 micron plasmid sisters in a tri-linked catenane complex.Nucleic acids research · 2010Article
- The selfish yeast plasmid uses the nuclear motor Kip1p but not Cin8p for its localization and equal segregation.The Journal of cell biology · 2009Article
- Meiotic transmission of an in vitro-assembled autonomous maize minichromosome.PLoS genetics · 2007Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The 2 microm circle plasmid in Saccharomyces cerevisiae is a model for a stable, high-copy-number, extrachromosomal "selfish" DNA element. By combining a partitioning system and an amplification system, the plasmid ensures its stable propagation and copy number maintenance, even though it does not provide any selective advantage to its host. Recent evidence suggests that the partitioning system couples plasmid segregation to chromosome segregation. We now demonstrate an unexpected and unconventional role for the mitotic spindle in the plasmid-partitioning pathway. The spindle specifies the nuclear address of the 2 microm circle and promotes recruitment of the cohesin complex to the plasmid-partitioning locus STB. Only the nuclear microtubules, and not the cytoplasmic ones, are required for loading cohesin at STB. In cells recovering from nocodazole-induced spindle depolymerization and G(2)/M arrest, cohesin-STB association can be established coincident with spindle restoration. This postreplication recruitment of cohesin is not functional in equipartitioning. However, normally acquired cohesin can be inactivated after replication without causing plasmid missegregation. In the mtw1-1 mutant yeast strain, the plasmid cosegregates with the spindle and the spindle-associated chromosomes; by contrast, a substantial number of the chromosomes are not associated with the spindle. These results are consistent with a model in which the spindle promotes plasmid segregation in a chromosome-linked fashion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.