ArticleMolecular and cellular biology2005
Phosphorylation by Cak1 regulates the C-terminal domain kinase Ctk1 in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 32 citations in OpenAlex.
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- Structure and activation mechanism of the yeast RNA Pol II CTD kinase CTDK-1 complex.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Neurospora RNA polymerase II kinase CTK negatively regulates catalase expression in a chromatin context-dependent manner.Environmental microbiology · 2020Article
- A SUMO-dependent pathway controls elongating RNA Polymerase II upon UV-induced damage.Scientific reports · 2019Article
- Gene modification by fast-track recombineering for cellular localization and isolation of components of plant protein complexes.The Plant journal : for cell and molecular biology · 2019Article
- Cdk7: a kinase at the core of transcription and in the crosshairs of cancer drug discovery.Transcription · 2019Review
- A feed forward circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation.Nucleic acids research · 2014Article
- Histone chaperones Nap1 and Vps75 regulate histone acetylation during transcription elongation.Molecular and cellular biology · 2013Article
- Article
- The CDK Network: Linking Cycles of Cell Division and Gene Expression.Genes & cancer · 2012Article
- Cdk9 T-loop phosphorylation is regulated by the calcium signaling pathway.Journal of cellular physiology · 2012Article
- Sporulation in the budding yeast Saccharomyces cerevisiae.Genetics · 2011Review
- Sub1 globally regulates RNA polymerase II C-terminal domain phosphorylation.Molecular and cellular biology · 2010Article
- A phosphorylation-independent role for the yeast cyclin-dependent kinase activating kinase Cak1.Gene · 2009Article
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- Article
- Pseudosubstrate inhibition of the anaphase-promoting complex by Acm1: regulation by proteolysis and Cdc28 phosphorylation.Molecular and cellular biology · 2008Article
- Article
- Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis.Molecular biology of the cell · 2007Article
- Mechanisms regulating the protein kinases of Saccharomyces cerevisiae.Eukaryotic cell · 2007Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Ctk1 is a Saccharomyces cerevisiae cyclin-dependent protein kinase (CDK) that assembles with Ctk2 and Ctk3 to form an active protein kinase complex, CTDK-I. CTDK-I phosphorylates Ser2 within the RNA polymerase II C-terminal domain, an activity that is required for efficient transcriptional elongation and 3' RNA processing. Ctk1 contains a conserved T loop, which undergoes activating phosphorylation in other CDKs. We show that Ctk1 is phosphorylated on Thr-338 within the T loop. Mutation of this residue abolished Ctk1 kinase activity in vitro and resulted in a cold-sensitive phenotype. As with other yeast CDKs undergoing T-loop phosphorylation, Ctk1 phosphorylation on Thr-338 was dependent on the Cak1 protein kinase. Ctk1 isolated from cak1Delta cells was unphosphorylated and exhibited low protein kinase activity. Moreover, Cak1 directly phosphorylated Ctk1 in vitro. Unlike wild-type cells, cells expressing Ctk1(T338A) delayed growth at early stationary phase, did not show the increase in Ser2 phosphorylation that normally accompanies the transition from rapid growth to stationary phase, and had compromised transcriptional activation of two stationary-phase genes, CTT1 and SPI1. Therefore, Ctk1 phosphorylation on Thr-338 is carried out by Cak1 and is required for normal gene transcription during the transition into stationary phase.
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