ArticleMolecular and cellular biology2005
The F-box protein Met30 is required for multiple steps in the budding yeast cell cycle.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Carboxy-Methylation of the Catalytic Subunit of Protein Phosphatase 2A (PP2Ac) Integrates Methionine Availability with Methionine Addicted Cancer Cell Proliferation.Biomolecules · 2025Article
- Evidence for a hydrogen sulfide-sensing E3 ligase in yeast.Genetics · 2024Article
- Molecular Biology of Cadmium Toxicity in Saccharomyces cerevisiae.Biological trace element research · 2021Review
- Sensing and Signaling of Methionine Metabolism.Metabolites · 2021Review
- Methionine Dependence of Cancer.Biomolecules · 2020Review
- Genome Wide Analysis of WD40 Proteins in Saccharomyces cerevisiae and Their Orthologs in Candida albicans.The protein journal · 2019Article
- S-adenosylmethionine limitation induces p38 mitogen-activated protein kinase and triggers cell cycle arrest in G1.Journal of cell science · 2014Article
- Downregulation of Cdc6 and pre-replication complexes in response to methionine stress in breast cancer cells.Cell cycle (Georgetown, Tex.) · 2012Article
- Characterizing the roles of Met31 and Met32 in coordinating Met4-activated transcription in the absence of Met30.Molecular biology of the cell · 2012Article
- Regulation of amino acid, nucleotide, and phosphate metabolism in Saccharomyces cerevisiae.Genetics · 2012Article
- A transcriptional activator is part of an SCF ubiquitin ligase to control degradation of its cofactors.Molecular cell · 2010Article
- Dissection of combinatorial control by the Met4 transcriptional complex.Molecular biology of the cell · 2010Article
- Lessons from fungal F-box proteins.Eukaryotic cell · 2009Review
- The emerging regulatory potential of SCFMet30 -mediated polyubiquitination and proteolysis of the Met4 transcriptional activator.Cell division · 2008Article
- A dominant suppressor mutation of the met30 cell cycle defect suggests regulation of the Saccharomyces cerevisiae Met4-Cbf1 transcription complex by Met32.The Journal of biological chemistry · 2008Article
- Nascent transcription of MCM2-7 is important for nuclear localization of the minichromosome maintenance complex in G1.Molecular biology of the cell · 2007Article
- Regulation of yeast oscillatory dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The yeast ubiquitin ligase SCFMet30: connecting environmental and intracellular conditions to cell division.Cell division · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Saccharomyces cerevisiae ubiquitin ligase SCF(Met30) is essential for cell cycle progression. To identify and characterize SCF(Met30)-dependent cell cycle steps, we used temperature-sensitive met30 mutants in cell cycle synchrony experiments. These experiments revealed a requirement for Met30 during both G(1)/S transition and M phase, while progression through S phase was unaffected by loss of Met30 function. Expression of the G(1)-specific transcripts CLN1, CLN2, and CLB5 was very low in met30 mutants, whereas expression of CLN3 was unaffected. However, overexpression of Cln2 could not overcome the G(1) arrest. Interestingly, overexpression of Clb5 could induce DNA replication in met30 mutants, albeit very inefficiently. Increased levels of Clb5 could not, however, suppress the cell proliferation defect of met30 mutants. Consistent with the DNA replication defects, chromatin immunoprecipitation experiments revealed significantly lower levels of the replication factors Mcm4, Mcm7, and Cdc45 at replication origins in met30 mutants than in wild-type cells. These data suggest that Met30 regulates several aspects of the cell cycle, including G(1)-specific transcription, initiation of DNA replication, and progression through M phase.
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