ArticleMolecular and cellular biology2005
The human beta-globin locus control region can silence as well as activate gene expression.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
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- Human Artificial Chromosome with Regulated Centromere: A Tool for Genome and Cancer Studies.ACS synthetic biology · 2018Review
- Developmental Switch in the Transcriptional Activity of a Long-Range Regulatory Element.Molecular and cellular biology · 2015Article
- A new light on DNA replication from the inactive X chromosome.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Review
- Complete TCR-α gene locus control region activity in T cells derived in vitro from embryonic stem cells.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Highly efficient site-specific transgenesis in cancer cell lines.Molecular cancer · 2012Article
- The chromatin backdrop of DNA replication: lessons from genetics and genome-scale analyses.Biochimica et biophysica acta · 2012Review
- Transcription factor binding at enhancers: shaping a genomic regulatory landscape in flux.Frontiers in genetics · 2012Article
- Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2.Molecular and cellular biology · 2011Article
- Article
- Transcription-dependent silencing of inducible convergent transgenes in transgenic mice.Epigenetics & chromatin · 2010Article
- Gene specificity of suppression of transgene-mediated insertional transcriptional activation by the chicken HS4 insulator.PloS one · 2009Article
- Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing.Genome research · 2009Article
- An iron responsive element-like stem-loop regulates alpha-hemoglobin-stabilizing protein mRNA.The Journal of biological chemistry · 2008Article
- Article
- Erythroid-specific expression of beta-globin by the sleeping beauty transposon for Sickle cell disease.Biochemistry · 2007Article
- Transcription of a productively rearranged Ig VDJC alpha does not require the presence of HS4 in the IgH 3' regulatory region.Journal of immunology (Baltimore, Md. : 1950) · 2007Article
- Expression of transgenes targeted to the Gt(ROSA)26Sor locus is orientation dependent.PloS one · 2006Article
- Histone H3 acetylation and H3 K4 methylation define distinct chromatin regions permissive for transgene expression.Molecular and cellular biology · 2006Article
- DNA methylation supports intrinsic epigenetic memory in mammalian cells.PLoS genetics · 2006Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Using recombinase-mediated cassette exchange to test multiple transgenes at the same site of integration, we demonstrate a novel chromatin context-dependent silencer activity of the beta-globin locus control region (LCR). This silencer activity requires DNase I hypersensitive sites HS2 and HS3 but not HS4. After silencing, the silenced cassettes adopt a typical closed chromatin conformation (histone H3 and H4 deacetylation, histone H3-K4 methylation, DNA methylation, and replication in late S phase). In the absence of the LCR at the same site of integration, the chromatin remains decondensed. We demonstrate that the LCR is necessary but not sufficient to trigger these chromatin changes. We also provide evidence that this novel silencing activity is caused by transcriptional interference triggered by activation of transcription in the flanking sequences by the LCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.