ArticleJournal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology2005
Cloning and characterization of SK2 channel from chicken short hair cells.
Article in Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Molecular Specializations Underlying Phenotypic Differences in Inner Ear Hair Cells of Zebrafish and Mice.bioRxiv : the preprint server for biology · 2024Article
- Molecular specializations underlying phenotypic differences in inner ear hair cells of zebrafish and mice.Frontiers in neurology · 2024Article
- Unraveling the Molecular Players at the Cholinergic Efferent Synapse of the Zebrafish Lateral Line.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- SK Current, Expressed During the Development and Regeneration of Chick Hair Cells, Contributes to the Patterning of Spontaneous Action Potentials.Frontiers in cellular neuroscience · 2021Article
- The Cholinergic Lateral Line Efferent Synapse: Structural, Functional and Molecular Similarities With Those of the Cochlea.Frontiers in cellular neuroscience · 2021Review
- Synaptic calcium regulation in hair cells of the chicken basilar papilla.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- A 'calcium capacitor' shapes cholinergic inhibition of cochlear hair cells.The Journal of physiology · 2014Review
- Alternative splice isoforms of small conductance calcium-activated SK2 channels differ in molecular interactions and surface levels.Channels (Austin, Tex.) · 2014Article
- Expression of the SK2 calcium-activated potassium channel is required for cholinergic function in mouse cochlear hair cells.The Journal of physiology · 2008Article
- Calcium-dependent inactivation of calcium channels in cochlear hair cells of the chicken.The Journal of physiology · 2007Article
- Ion channel gene expression in the inner ear.Journal of the Association for Research in Otolaryngology : JARO · 2007Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
In the inner ear of birds, as in mammals, reptiles and amphibians, acetylcholine released from efferent neurons inhibits hair cells via activation of an apamin-sensitive, calcium-dependent potassium current. The particular potassium channel involved in avian hair cell inhibition is unknown. In this study, we cloned a small-conductance, calcium-sensitive potassium channel (gSK2) from a chicken cochlear library. Using RT-PCR, we demonstrated the presence of gSK2 mRNA in cochlear hair cells. Electrophysiological studies on transfected HEK293 cells showed that gSK2 channels have a conductance of approximately 16 pS and a half-maximal calcium activation concentration of 0.74+/-0.17 microM. The expressed channels were blocked by apamin (IC(50)=73.3+/-5.0 pM) and d-tubocurarine (IC(50)=7.6+/-1.0 microM), but were insensitive to charybdotoxin. These characteristics are consistent with those reported for acetylcholine-induced potassium currents of isolated chicken hair cells, suggesting that gSK2 is involved in efferent inhibition of chicken inner ear. These findings imply that the molecular mechanisms of inhibition are conserved in hair cells of all vertebrates.
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