ArticleJournal of bacteriology2005
Transcription activation in vitro by the Bradyrhizobium japonicum regulatory protein FixK2.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Soybean-mediated suppression of BjaI/BjaRApplied and environmental microbiology · 2024Article
- Photoacoustic Calorimetry Studies of OBiochemistry · 2024Article
- Fine-Tuning Modulation of Oxidation-Mediated Posttranslational Control ofInternational journal of molecular sciences · 2022Article
- Regulation of the Emissions of the Greenhouse Gas Nitrous Oxide by the Soybean EndosymbiontInternational journal of molecular sciences · 2022Article
- Multiple sensors provide spatiotemporal oxygen regulation of gene expression in a Rhizobium-legume symbiosis.PLoS genetics · 2021Article
- An Integrated Systems Approach Unveils New Aspects of Microoxia-Mediated Regulation inFrontiers in microbiology · 2019Article
- Expanding the Regulon of theFrontiers in microbiology · 2019Article
- FixKFrontiers in microbiology · 2017Article
- Nitrogen Fixation and Molecular Oxygen: Comparative Genomic Reconstruction of Transcription Regulation in Alphaproteobacteria.Frontiers in microbiology · 2016Article
- The global response regulator RegR controls expression of denitrification genes in Bradyrhizobium japonicum.PloS one · 2014Article
- The structure of Bradyrhizobium japonicum transcription factor FixK2 unveils sites of DNA binding and oxidation.The Journal of biological chemistry · 2013Article
- Bacterial adaptation of respiration from oxic to microoxic and anoxic conditions: redox control.Antioxidants & redox signaling · 2012Review
- Article
- Genome-wide transcriptional and physiological responses of Bradyrhizobium japonicum to paraquat-mediated oxidative stress.Applied and environmental microbiology · 2011Article
- Regulation of the phototrophic iron oxidation (pio) genes in Rhodopseudomonas palustris TIE-1 is mediated by the global regulator, FixK.Molecular microbiology · 2011Article
- Reconstruction of the core and extended regulons of global transcription factors.PLoS genetics · 2010Article
- Autoregulation of fixK(2) gene expression in Bradyrhizobium japonicum.Molecular genetics and genomics : MGG · 2010Article
- Posttranslational control of transcription factor FixK2, a key regulator for the Bradyrhizobium japonicum-soybean symbiosis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Comprehensive assessment of the regulons controlled by the FixLJ-FixK2-FixK1 cascade in Bradyrhizobium japonicum.Journal of bacteriology · 2008Article
- New target genes controlled by the Bradyrhizobium japonicum two-component regulatory system RegSR.Journal of bacteriology · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Bradyrhizobium japonicum, the N2-fixing root nodule endosymbiont of soybean, a group of genes required for microaerobic, anaerobic, or symbiotic growth is controlled by FixK2, a key regulator that is part of the FixLJ-FixK2 cascade. FixK2 belongs to the family of cyclic AMP receptor protein/fumarate and nitrate reductase (CRP/FNR) transcription factors that recognize a palindromic DNA motif (CRP/FNR box) associated with the regulated promoters. Here, we report on a biochemical analysis of FixK2 and its transcription activation activity in vitro. FixK2 was expressed in Escherichia coli and purified as a soluble N-terminally histidine-tagged protein. Gel filtration experiments revealed that increasing the protein concentration shifts the monomer-dimer equilibrium toward the dimer. Purified FixK2 productively interacted with the B. japonicum sigma80-RNA polymerase holoenzyme, but not with E. coli sigma70-RNA polymerase holoenzyme, to activate transcription from the B. japonicum fixNOQP, fixGHIS, and hemN2 promoters in vitro. Furthermore, FixK2 activated transcription from the E. coli FF(-41.5) model promoter, again only in concert with B. japonicum RNA polymerase. All of these promoters are so-called class II CRP/FNR-type promoters. We showed by specific mutagenesis that the FixK2 box at nucleotide position -40.5 in the hemN2 promoter, but not that at -78.5, is crucial for activation both in vivo and in vitro, which argues against recognition of a potential class III promoter. Given the lack of any evidence for the presence of a cofactor in purified FixK2, we surmise that FixK2 alone is sufficient to activate in vitro transcription to at least a basal level. This contrasts with all well-studied CRP/FNR-type proteins, which do require coregulators.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.