ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
DNA hypomethylation in 5-azacytidine-induced early-flowering lines of flax.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 83 citations in OpenAlex.
- Understanding epigenetic regulation in nonmodel plants: transcriptomic responses to seed demethylation in leaves and roots of the annual herb Erodium cicutarium (Geraniaceae).G3 (Bethesda, Md.) · 2026Article
- Transgenerational genetic and epigenetic changes induced by gamma-ray in Fagopyrum species.BMC plant biology · 2025Article
- Demethylating drugs alter protoplast development, regeneration, and the genome stability of protoplast-derived regenerants of cabbage.BMC plant biology · 2025Article
- Role of methylation in vernalization and photoperiod pathway: a potential flowering regulator?Horticulture research · 2023Article
- Comparative analysis of the chrysanthemum transcriptome with DNA methylation inhibitors treatment and silencing MET1 lines.BMC plant biology · 2023Article
- CRISPR/Cas9-targeted mutagenesis ofHorticulture research · 2022Article
- Epigenetic memories and the evolution of infectious diseases.Nature communications · 2021Article
- Exploration of Epigenetics for Improvement of Drought and Other Stress Resistance in Crops: A Review.Plants (Basel, Switzerland) · 2021Review
- Experimental DNA Demethylation Associates with Changes in Growth and Gene Expression of Oak Tree Seedlings.G3 (Bethesda, Md.) · 2020Article
- Target of Rapamycin Regulates Genome Methylation Reprogramming to Control Plant Growth inFrontiers in genetics · 2020Article
- Rapid changes in seed dispersal traits may modify plant responses to global change.AoB PLANTS · 2019Review
- Photoperiod sensitivity of Canadian flax cultivars and 5-azacytidine treated early flowering derivative lines.BMC plant biology · 2019Article
- Article
- 5-Azacytidine treatment and TaPBF-D over-expression increases glutenin accumulation within the wheat grain by hypomethylating the Glu-1 promoters.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- 5-Azacytidine mediated reactivation of silenced transgenes in potato (Solanum tuberosum) at the whole plant level.Plant cell reports · 2017Article
- Concerted Flexibility of Chromatin Structure, Methylome, and Histone Modifications along with Plant Stress Responses.Biology · 2017Review
- Quantitative trait variation is revealed in a novel hypomethylated population of woodland strawberry (Fragaria vesca).BMC plant biology · 2016Article
- Evidence for an epigenetic role in inbreeding depression.Biology letters · 2012Article
- Changes in micro RNA expression in a wild tuber-bearing Solanum species induced by 5-Azacytidine treatment.Plant cell reports · 2012Article
- DNA methylation patterns of Brachypodium distachyon chromosomes and their alteration by 5-azacytidine treatment.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
HPLC analysis was used to examine the cytosine methylation of total DNA extracted from four early-flowering lines that were induced by treating germinating seeds of flax (Linum usitatissimum) with the DNA demethylating agent 5-azacytidine. In the normal lines that gave rise to the induced early-flowering lines, flowering usually begins approximately 50 days after sowing. The early-flowering lines flower 7-13 days earlier than normal. The normal level of cytosine methylation was approximately 14% of the cytosines and 2.7% of the nucleosides. In the early-flowering lines, these levels were 6.2% lower than normal in DNA from the terminal leaf clusters of 14-day-old seedlings and 9.7% lower than normal in DNA from the cotyledons and immature shoot buds of 4-day-old seedlings. This hypomethylation was seen in lines that were five to nine generations beyond the treatment generation. The level of hypomethylation was similar in three of the four early-flowering lines, but was not as low in the fourth line, which flowers early but not quite as early as the other three lines. Unexpectedly, the degree of hypomethylation seen in segregant lines, derived by selecting for the early-flowering phenotype in the F(2) and F(3) generations of out-crosses, was similar to that seen in the early-flowering lines. Analysis of the methylation levels in segregating generations of out-crosses between early-flowering and normal lines demonstrated a decrease in methylation level during the selection of early-flowering segregants. The results suggest an association between hypomethylation and the early-flowering phenotype, and that the hypomethylated regions may not be randomly distributed throughout the genome of the early-flowering lines.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.