ArticleMarine biotechnology (New York, N.Y.)
Induction of Japanese flounder tnf promoter activity by lipopolysaccharide in zebrafish embryo.
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Article
- LPS response and tolerance in the zebrafish (Danio rerio).Fish & shellfish immunology · 2009Article
- Transgenic zebrafish expressing chicken lysozyme show resistance against bacterial diseases.Transgenic research · 2006Article
- Efficient transfection strategy for the spatiotemporal control of gene expression in zebrafish.Marine biotechnology (New York, N.Y.)Article
- Upstream regulatory region of zebrafish lunatic fringe: isolation and promoter analysis.Marine biotechnology (New York, N.Y.)Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A recombinant plasmid containing the 2381-bp promoter region of Japanese flounder tumor necrosis factor (TNF) and green fluorescence protein (GFP) was introduced into zebrafish fertilized eggs by microinjection. GFP was expressed in 2 transgenic zebrafish lines in the heart and around the pharynx under unstressed condition. When embryos were exposed to lipopolysaccharide (LPS), GFP was expressed in the whole zebrafish embryonic surface, and Western blot analysis also showed that the level of the expressed GFP protein in zebrafish embryo was higher at an LPS concentration of 140 microg/ml than at LPS concentrations of 0 and 70 microg/ml. Stimulation with either concanavalin A or phorbol myristate acetate induced weak GFP expression, but stimulation with both of them induced strong expression similar to that induced by LPS. GFP expression peaked 1 hour after stimulation, then gradually decreased. These results indicate that transcription regulated by the Japanese flounder TNF promoter could be under the control of the LPS-recognition system in zebrafish embryos.
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