ArticlePlant physiology2005
Molecular and biochemical characterization of the selenocysteine Se-methyltransferase gene and Se-methylselenocysteine synthesis in broccoli.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 145 citations in OpenAlex.
- Article
- Enhanced selenocysteine biosynthesis for seleno-methylselenocysteine production in Bacillus subtilis.Applied microbiology and biotechnology · 2023Article
- iTRAQ-Based Proteomic Analyses of Regulation of Isothiocyanate and Endogenous Selenium Metabolism in Broccoli Sprouts by Exogenous Sodium Selenite.Foods (Basel, Switzerland) · 2023Article
- Effect of Sodium Selenate and Selenocystine on Savoy Cabbage Yield, Morphological and Biochemical Characteristics underPlants (Basel, Switzerland) · 2023Article
- Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.Molecules (Basel, Switzerland) · 2022Review
- Selenium Uptake, Transport, Metabolism, Reutilization, and Biofortification in Rice.Rice (New York, N.Y.) · 2022Review
- Seleno-Amino Acids in Vegetables: A Review of Their Forms and Metabolism.Frontiers in plant science · 2022Review
- A Novel mRNA-Mediated and MicroRNA-Guided Approach to Specifically Eradicate Drug-Resistant Hepatocellular Carcinoma Cell Lines by Se-Methylselenocysteine.Antioxidants (Basel, Switzerland) · 2021Article
- Selenium transport and metabolism in plants: Phytoremediation and biofortification implications.Journal of hazardous materials · 2021Review
- Phosphorus and sulfur in a tropical soil and their effects on growth and selenium accumulation in Leucaena leucocephala (Lam.) de Wit.Environmental science and pollution research international · 2020Article
- Selenite Uptake and Transformation in Rice Seedlings (Frontiers in plant science · 2020Article
- Selenium Application During Radish (Plants (Basel, Switzerland) · 2019Article
- Comparative transcriptome analysis of two selenium-accumulating genotypes of Aegilops tauschii Coss. in response to selenium.BMC genetics · 2019Article
- Cytokinin is involved in TPS22-mediated selenium tolerance in Arabidopsis thaliana.Annals of botany · 2018Article
- Mechanisms of Selenium Enrichment and Measurement in Brassicaceous Vegetables, and Their Application to Human Health.Frontiers in plant science · 2017Review
- Selenium-Induced Toxicity Is Counteracted by Sulfur in Broccoli (Frontiers in plant science · 2017Article
- Plastid ribosomal protein S5 is involved in photosynthesis, plant development, and cold stress tolerance in Arabidopsis.Journal of experimental botany · 2016Article
- Selenium accumulation by plants.Annals of botany · 2016Review
- Selenium species in the roots and shoots of chickpea plants treated with different concentrations of sodium selenite.Environmental science and pollution research international · 2015Article
- Effects of selenium biofortification on crop nutritional quality.Frontiers in plant science · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Selenium (Se) plays an indispensable role in human nutrition and has been implicated to have important health benefits, including being a cancer preventative agent. While different forms of Se vary in their anticarcinogenic efficacy, Se-methylselenocysteine (SeMSC) has been demonstrated to be one of the most effective chemopreventative compounds. Broccoli (Brassica oleracea var. italica) is known for its ability to accumulate high levels of Se with the majority of the selenoamino acids in the form of Se-methylselenocysteine. Therefore, it serves as a good model to study the regulation of SeMSC accumulation in plants. A cDNA encoding selenocysteine Se-methyltransferase, the key enzyme responsible for SeMSC formation, was cloned from broccoli using a homocysteine S-methyltransferase gene probe from Arabidopsis (Arabidopsis thaliana). This clone, designated as BoSMT, was functionally expressed in Escherichia coli, and its identity was confirmed by its substrate specificity in the methylation of selenocysteine. The BoSMT gene represents a single copy sequence in the broccoli genome. Examination of BoSMT gene expression and SeMSC accumulation in response to selenate, selenite, and sulfate treatments showed that the BoSMT transcript and SeMSC synthesis were significantly up-regulated in plants exposed to selenate but were low in plants supplied with selenite. Simultaneous treatment of selenate with selenite significantly reduced SeMSC production. In addition, high levels of sulfate suppressed selenate uptake, resulting in a dramatic reduction of BoSMT mRNA level and SeMSC accumulation. Our results reveal that SeMSC accumulation closely correlated with the BoSMT gene expression and the total Se status in tissues and provide important information for maximizing the SeMSC production in this beneficial vegetable plant.
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