ArticleJournal of cell science2005
Grp/DChk1 is required for G2-M checkpoint activation in Drosophila S2 cells, whereas Dmnk/DChk2 is dispensable.
Article in Journal of cell science, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
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- Transcriptional signature of prion-induced neurotoxicity in a Drosophila model of transmissible mammalian prion disease.The Biochemical journal · 2020Article
- Overexpression of theHereditas · 2018Article
- Alternative Chk1-independent S/M checkpoint in somatic cells that prevents premature mitotic entry.Medical oncology (Northwood, London, England) · 2017Article
- ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association.The EMBO journal · 2011Article
- Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1.Molecular biology of the cell · 2011Article
- Identification of dAven, a Drosophila melanogaster ortholog of the cell cycle regulator Aven.Cell cycle (Georgetown, Tex.) · 2011Article
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- A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint.Science signaling · 2011Article
- Pantethine rescues a Drosophila model for pantothenate kinase-associated neurodegeneration.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
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- Stwl modifies chromatin compaction and is required to maintain DNA integrity in the presence of perturbed DNA replication.Molecular biology of the cell · 2009Article
- Telomere loss provokes multiple pathways to apoptosis and produces genomic instability in Drosophila melanogaster.Genetics · 2008Article
- A long-term flow cytometry assay to analyze the role of specific genes of Drosophila melanogaster S2 cells in surviving genotoxic stress.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2008Article
- Reducing DNA polymerase alpha in the absence of Drosophila ATR leads to P53-dependent apoptosis and developmental defects.Genetics · 2007Article
- Drosophila ATR in double-strand break repair.Genetics · 2007Article
- Functional differentiation of SWI/SNF remodelers in transcription and cell cycle control.Molecular and cellular biology · 2007Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Cell-cycle checkpoints are signal-transduction pathways required to maintain genomic stability in dividing cells. Previously, it was reported that two kinases essential for checkpoint signalling, Chk1 and Chk2 are structurally conserved. In contrast to yeast, Xenopus and mammals, the Chk1- and Chk2-dependent pathways in Drosophila are not understood in detail. Here, we report the function of these checkpoint kinases, referred to as Grp/DChk1 and Dmnk/DChk2 in Drosophila Schneider's cells, and identify an upstream regulator as well as downstream targets of Grp/DChk1. First, we demonstrate that S2 cells are a suitable model for G(2)/M checkpoint studies. S2 cells display Grp/DChk1-dependent and Dmnk/DChk2-independent cell-cycle-checkpoint activation in response to hydroxyurea and ionizing radiation. S2 cells depleted for Grp/DChk1 using RNA interference enter mitosis in the presence of impaired DNA integrity, resulting in prolonged mitosis and mitotic catastrophe. Grp/DChk1 is phosphorylated in a Mei-41/DATR-dependent manner in response to hydroxyurea and ionizing radiation, indicating that Mei-41/ATR is an upstream component in the Grp/DChk1 DNA replication and DNA-damage-response pathways. The level of Cdc25(Stg) and phosphorylation status of Cdc2 are modulated in a Grp/DChk1-dependent manner in response to hydroxyurea and irradiation, indicating that these cell-cycle regulators are downstream targets of the Grp/DChk1-dependent DNA replication and DNA-damage responses. By contrast, depletion of Dmnk/DChk2 by RNA interference had little effect on checkpoint responses to hydroxyurea and irradiation. We conclude that Grp/DChk1, and not Dmnk/DChk2, is the main effector kinase involved in G(2)/M checkpoint control in Drosophila cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.