ArticleThe Journal of physiology2005
Biophysical and pharmacological characterization of nicotinic cholinergic receptors in rat cochlear inner hair cells.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 99 citations in OpenAlex.
- Efferent Inhibition of Hair Cells: Past, Present and Future.Journal of the Association for Research in Otolaryngology : JARO · 2026Review
- Impaired Development of the Medial Olivocochlear System in a KCNQ4-Deficient Mouse Model.Molecular neurobiology · 2026Article
- Co-release of GABA and ACh from Medial Olivocochlear Neurons as a Fine Regulatory Mechanism of Cochlear Efferent Inhibition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Strengthening Medial Olivocochlear Feedback Reduces the Developmental Impact of Early Noise Exposure.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Strengthening Medial Olivocochlear Feedback Reduces the Developmental Impact of Early Noise Exposure.bioRxiv : the preprint server for biology · 2025Article
- In vivo AAV9-Myo7a gene rescue restores hearing and cholinergic efferent innervation in inner hair cells.JCI insight · 2024Article
- Co-release of GABA and ACh from medial olivocochlear neurons fine tunes cochlear efferent inhibition.bioRxiv : the preprint server for biology · 2024Article
- Key role of the TM2-TM3 loop in calcium potentiation of the α9α10 nicotinic acetylcholine receptor.Cellular and molecular life sciences : CMLS · 2024Article
- The Remarkable Outer Hair Cell: Proceedings of a Symposium in Honour of W. E. Brownell.Journal of the Association for Research in Otolaryngology : JARO · 2023Review
- Recent Advances in the Discovery of Nicotinic Acetylcholine Receptor Allosteric Modulators.Molecules (Basel, Switzerland) · 2023Review
- The α9α10 nicotinic acetylcholine receptor: a compelling drug target for hearing loss?Expert opinion on therapeutic targets · 2022Review
- Efferent neurons control hearing sensitivity and protect hearing from noise through the regulation of gap junctions between cochlear supporting cells.Journal of neurophysiology · 2022Article
- Synaptic Contributions to Cochlear Outer Hair Cell CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Unraveling the Molecular Players at the Cholinergic Efferent Synapse of the Zebrafish Lateral Line.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Characterization of Developmental Changes in Spontaneous Electrical Activity of Medial Superior Olivary Neurons Before Hearing Onset With a Combination of Injectable and Volatile Anesthesia.Frontiers in neuroscience · 2021Article
- Loss of Choline Agonism in the Inner Ear Hair Cell Nicotinic Acetylcholine Receptor Linked to the α10 Subunit.Frontiers in molecular neuroscience · 2021Article
- The Hair Cell α9α10 Nicotinic Acetylcholine Receptor: Odd Cousin in an Old Family.Frontiers in cellular neuroscience · 2021Review
- The Cholinergic Lateral Line Efferent Synapse: Structural, Functional and Molecular Similarities With Those of the Cochlea.Frontiers in cellular neuroscience · 2021Review
- Nicotine evoked efferent transmitter release onto immature cochlear inner hair cells.Journal of neurophysiology · 2020Article
- Functional Postnatal Maturation of the Medial Olivocochlear Efferent-Outer Hair Cell Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Before the onset of hearing, a transient efferent innervation is found on inner hair cells (IHCs). This synapse is inhibitory and mediated by a nicotinic cholinergic receptor (nAChR) probably formed by the alpha9 and alpha10 subunits. We analysed the pharmacological and biophysical characteristics of the native nAChR using whole-cell recordings from IHCs in acutely excised apical turns of the rat organ of Corti. Nicotine did not activate but rather blocked the acetylcholine (ACh)-evoked currents with an IC50 of 1 +/- 0.1 microM. Antagonists of non-cholinergic receptors such as strychnine, bicuculline and ICS-205930 blocked ACh-evoked responses with an IC50 of 8.6 +/- 0.8 nM, 59 +/- 4 nM and 0.30 +/- 0.02 microM, respectively. The IHC nAChR was both permeable to (P(Ca)/P(Na) = 8 +/- 0.9) and modulated by external Ca2+. ACh-evoked currents were potentiated by Ca2+ up to 500 microM but were reduced by higher concentrations of this cation. Ba2+ mimicked the effects of Ca2+ whereas Mg2+ only blocked these currents. In addition, elevation of extracellular Ca2+ reduced the amplitude of spontaneous synaptic currents without affecting their time course. The receptor had an EC50 for ACh of 60.7 +/- 2.8 microM in 0.5 mM Ca2+. In the absence of Ca2+, the EC50 for ACh increased, suggesting that potentiation by Ca2+ involves changes in the apparent affinity for the agonist. These pharmacological and biophysical characteristics of the IHC nAChR closely resemble those of the recombinant alpha9alpha10 nAChR, reinforcing the hypothesis that the functional nAChR at the olivocochlear efferent-IHC synapse is composed of both the alpha9 and alpha10 subunits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.