ArticleJournal of virology2005
The polypyrimidine tract binding protein is required for efficient picornavirus gene expression and propagation.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- Dancing with the Devil: A Review of the Importance of Host RNA-Binding Proteins to Alphaviral RNAs during Infection.Viruses · 2023Review
- Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients.International journal of biological sciences · 2023Article
- Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLCell reports · 2021Article
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- The long-lasting enigma of polycytidine (polyC) tract.PLoS pathogens · 2021Review
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- Stress proteins: the biological functions in virus infection, present and challenges for target-based antiviral drug development.Signal transduction and targeted therapy · 2020Review
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- Human astroviruses: in silico analysis of the untranslated region and putative binding sites of cellular proteins.Molecular biology reports · 2019Review
- The Golgi associated ERI3 is a Flavivirus host factor.Scientific reports · 2016Article
- The role of Misshapen NCK-related kinase (MINK), a novel Ste20 family kinase, in the IRES-mediated protein translation of human enterovirus 71.PLoS pathogens · 2015Article
- The role of viral persistence in flavivirus biology.Pathogens and disease · 2014Review
- Article
- New insights into functional roles of the polypyrimidine tract-binding protein.International journal of molecular sciences · 2013Review
- Thiouracil cross-linking mass spectrometry: a cell-based method to identify host factors involved in viral amplification.Journal of virology · 2013Article
- Article
- Enterovirus type 71 2A protease functions as a transcriptional activator in yeast.Journal of biomedical science · 2010Article
- Insights into the biology of IRES elements through riboproteomic approaches.Journal of biomedicine & biotechnology · 2010Review
- Polypyrimidine tract-binding protein influences negative strand RNA synthesis of dengue virus.Biochemical and biophysical research communications · 2009Article
- The polypyrimidine tract-binding protein is required for efficient dengue virus propagation and associates with the viral replication machinery.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Mammalian host factors required for efficient viral gene expression and propagation have been often recalcitrant to genetic analysis. A case in point is the function of cellular factors that trans-activate internal ribosomal entry site (IRES)-driven translation, which is operative in many positive-stranded RNA viruses, including all picornaviruses. These IRES trans-acting factors have been elegantly studied in vitro, but their in vivo importance for viral gene expression and propagation has not been widely confirmed experimentally. Here we use RNA interference to deplete mammalian cells of one such factor, the polypyrimidine tract binding protein, and test its requirement in picornavirus gene expression and propagation. Depletion of the polypyrimidine tract binding protein resulted in a marked delay of particle propagation and significantly decreased synthesis and accumulation of viral proteins of poliovirus and encephalomyocarditis virus. These effects could be partially restored by expression of an RNA interference-resistant exogenous polypyrimidine tract binding protein. These data indicate a critical role for the polypyrimidine tract binding protein in picornavirus gene expression and strongly suggest a requirement for efficient IRES-dependent translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.