ArticleMolecular biology of the cell2005
Pushing forces drive the comet-like motility of microtubule arrays in Dictyostelium.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Actin network architecture can ensure robust centering or sensitive decentering of the centrosome.The EMBO journal · 2022Article
- Microtubule-dependent pushing forces contribute to long-distance aster movement and centration inMolecular biology of the cell · 2020Article
- Centering and Shifting of Centrosomes in Cells.Cells · 2020Review
- Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation.Cell reports · 2020Article
- Bioenergetics of theBiomolecules · 2020Article
- Review
- Force balances between interphase centrosomes as revealed by laser ablation.Molecular biology of the cell · 2019Article
- Review
- Centrosome centering and decentering by microtubule network rearrangement.Molecular biology of the cell · 2016Article
- Shape-motion relationships of centering microtubule asters.The Journal of cell biology · 2016Article
- Organization of microtubule assemblies in Dictyostelium syncytia depends on the microtubule crosslinker, Ase1.Cellular and molecular life sciences : CMLS · 2016Article
- Diagnostic Estimation of Noninvasive Tests for Hepatic Fibrosis in Chronic Hepatitis B Patients Without a Gold Standard.Hepatitis monthly · 2016Article
- Lateral motion and bending of microtubules studied with a new single-filament tracking routine in living cells.Biophysical journal · 2014Article
- A kinesin-mediated mechanism that couples centrosomes to nuclei.Cellular and molecular life sciences : CMLS · 2013Article
- Article
- Functional characterization of CP148, a novel key component for centrosome integrity in Dictyostelium.Cellular and molecular life sciences : CMLS · 2012Article
- The branched actin nucleator Arp2/3 promotes nuclear migrations and cell polarity in the C. elegans zygote.Developmental biology · 2011Article
- Analysis of Dictyostelium TACC reveals differential interactions with CP224 and unusual dynamics of Dictyostelium microtubules.Cellular and molecular life sciences : CMLS · 2011Article
- Finding the cell center by a balance of dynein and myosin pulling and microtubule pushing: a computational study.Molecular biology of the cell · 2010Article
- Anterograde microtubule transport drives microtubule bending in LLC-PK1 epithelial cells.Molecular biology of the cell · 2009Article
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Authors and funding
6 authors.
Funding
Abstract
Overexpression of dynein fragments in Dictyostelium induces the movement of the entire interphase microtubule array. Centrosomes in these cells circulate through the cytoplasm at rates between 0.4 and 2.5 microm/s and are trailed by a comet-tail like arrangement of the microtubule array. Previous work suggested that these cells use a dynein-mediated pulling mechanism to generate this dramatic movement and that similar forces are at work to maintain the interphase MTOC position in wild-type cells. In the present study, we address the nature of the forces used to produce microtubule movement. We have used a laser microbeam to sever the connection between the motile centrosomes and trailing microtubules, demonstrating that the major force for such motility results from a pushing on the microtubules. We eliminate the possibility that microtubule assembly/disassembly reactions are significant contributors to this motility and suggest that the cell cortex figures prominently in locating force-producing molecules. Our findings indicate that interphase microtubules in Dictyostelium are subject to both dynein- and kinesin-like forces and that these act in concert to maintain centrosome position in the cell and to support the radial character of the microtubule network.
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