ArticleRespiratory research2005
Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells.
Article in Respiratory research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
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- Cryopreserved biopsy tissues of rectal cancer liver metastasis for assessment of anticancer drug response in vitro and in vivo.Oncology reports · 2020Article
- Influenza-mediated reduction of lung epithelial ion channel activity leads to dysregulated pulmonary fluid homeostasis.JCI insight · 2018Article
- X-ray radiation-induced and targeted photodynamic therapy with folic acid-conjugated biodegradable nanoconstructs.International journal of nanomedicine · 2018Article
- Resolution of pulmonary edema. Thirty years of progress.American journal of respiratory and critical care medicine · 2014Article
- Cytokine and chemokine responses of lung exposed to surrogate viral and bacterial infections.Comparative medicine · 2013Article
- Tissue protection and endothelial cell signaling by 20-HETE analogs in intact ex vivo lung slices.Experimental cell research · 2012Article
- Exploring lung physiology in health and disease with lung slices.Pulmonary pharmacology & therapeutics · 2011Review
- Enhancement of alveolar epithelial sodium channel activity with decreased cystic fibrosis transmembrane conductance regulator expression in mouse lung.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Alveolar epithelial CNGA1 channels mediate cGMP-stimulated, amiloride-insensitive, lung liquid absorption.Pflugers Archiv : European journal of physiology · 2011Article
- Ion transport by pulmonary epithelia.Journal of biomedicine & biotechnology · 2011Review
- In vitro transdifferentiation of human fetal type II cells toward a type I-like cell.Pediatric research · 2007Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically, the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the alveolar type I cell might significantly contribute to the overall ion and fluid homeostasis of the lung, direct assessment of functional ion channels in type I cells has remained elusive.
methodsHere we describe a development of a lung slice preparation that has allowed positive identification of alveolar type I cells within an intact and viable alveolar epithelium using living cell immunohistochemistry.
resultsThis technique has allowed, for the first time, single ion channels of identified alveolar type I cells to be recorded using the cell-attached configuration of the patch-clamp technique.
conclusionThis exciting new development should facilitate the ascription of function to alveolar type I cells and allow us to integrate this cell type into the general model of alveolar ion and fluid balance in health and disease.
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