ArticleHistochemistry and cell biology2005
Distribution of caveolin-1 and connexin43 in normal and injured alveolar epithelial R3/1 cells.
Article in Histochemistry and cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- Selective inhibition of Cx43 hemichannels by Gap19 and its impact on myocardial ischemia/reperfusion injury.Basic research in cardiology · 2013Article
- Caveolin-1 modulates TGF-β1 signaling in cardiac remodeling.Matrix biology : journal of the International Society for Matrix Biology · 2011Article
- Spontaneous lung dysfunction and fibrosis in mice lacking connexin 40 and endothelial cell connexin 43.The American journal of pathology · 2011Article
- Compartmentalized connexin 43 s-nitrosylation/denitrosylation regulates heterocellular communication in the vessel wall.Arteriosclerosis, thrombosis, and vascular biology · 2011Article
- Regulation of gap junctions in porcine cumulus-oocyte complexes: contributions of granulosa cell contact, gonadotropins, and lipid rafts.Molecular endocrinology (Baltimore, Md.) · 2009Article
- Gap junctions in the control of vascular function.Antioxidants & redox signaling · 2009Review
- Caveolin-1 and -2 interact with connexin43 and regulate gap junctional intercellular communication in keratinocytes.Molecular biology of the cell · 2008Article
- The histochemistry and cell biology vade mecum: a review of 2005-2006.Histochemistry and cell biology · 2006Review
- Lack of evidence for caveolin-1 and CD147 interaction before and after bleomycin-induced lung injury.Histochemistry and cell biology · 2006Article
- Pathways and control of connexin oligomerization.Trends in cell biology · 2006Review
- Recent progress in histochemistry and cell biology: the state of the art 2005.Histochemistry and cell biology · 2005Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using the new alveolar epithelial type I-like cell line R3/1 derived from fetal rat lung, we studied the distribution of connexin43 and caveolin-1 under conditions of bleomycin-induced injury in vitro. We show that under normal as well as under conditions of injury, endogenous connexin43 does not directly interact with endogenous caveolin-1 as revealed by immunofluorescence, glutathione S-transferase/caveolin-1 "pull down" assay, and co-immunoprecipitation experiments. The assessment of Triton X-100 solubility revealed that caveolin-1 was abundant in detergent-resistant membrane fractions. This is consistent with the localization of caveolin-1 in the lipid rafts/caveolae. Similarly, phosphorylated connexin43 was preferably detected in the Triton-insoluble fraction. Using a sucrose gradient we demonstrated that the majority of phosphorylated connexin43 colocalizes with caveolin-1 in lipid rafts, whereas all other forms of connexin43 remain in the bulk of cellular membranes and cytosolic proteins. Triton solubility assessment of bleomycin-treated cells revealed no differences in the caveolin-1 and connexin43 distribution. A further interesting outcome of our study is the shift of caveolin-1 from the lipid raft/caveolae fractions to the non-caveolar fractions after bleomycin treatment indicating an intracellular retention of caveolin-1. This result suggests the possibility that the translocation of caveolin-1 could be an important event regulating the metabolism of alveolar epithelial lung cells after injury.
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