ArticleMolecular microbiology2005
Measuring chromosome dynamics on different time scales using resolvases with varying half-lives.
Article in Molecular microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed.
- Bacterial nucleoid is a riddle wrapped in a mystery inside an enigma.Journal of bacteriology · 2024Review
- Catastrophic chromosome fragmentation probes the nucleoid structure and dynamics in Escherichia coli.Nucleic acids research · 2022Article
- Spatiotemporal Coupling of DNA Supercoiling and Genomic Sequence Organization-A Timing Chain for the Bacterial Growth Cycle?Biomolecules · 2022Review
- Extended sister-chromosome catenation leads to massive reorganization of the E. coli genome.Nucleic acids research · 2022Article
- Elucidating the Influence of Chromosomal Architecture on Transcriptional Regulation in Prokaryotes - Observing Strong Local Effects of Nucleoid Structure on Gene Regulation.Frontiers in microbiology · 2020Article
- Supercoil Levels inMicroorganisms · 2019Article
- Enteropathogens: Tuning Their Gene Expression for Hassle-Free Survival.Frontiers in microbiology · 2018Review
- Species-specific supercoil dynamics of the bacterial nucleoid.Biophysical reviews · 2016Article
- Large-Scale Conformational Transitions in Supercoiled DNA Revealed by Coarse-Grained Simulation.Biophysical journal · 2016Article
- Organization of DNA in a bacterial nucleoid.BMC microbiology · 2016Article
- Topological Behavior of Plasmid DNA.Microbiology spectrum · 2015Review
- Bacterial chromosome organization and segregation.Annual review of cell and developmental biology · 2015Review
- New approaches to understanding the spatial organization of bacterial genomes.Current opinion in microbiology · 2014Review
- RNA polymerase: chromosome domain boundary maker and regulator of supercoil density.Current opinion in microbiology · 2014Review
- Quantitation of the DNA tethering effect in long-range DNA looping in vivo and in vitro using the Lac and λ repressors.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Transposable prophage Mu is organized as a stable chromosomal domain of E. coli.PLoS genetics · 2013Article
- Computational analyses of transcriptomic data reveal the dynamic organization of the Escherichia coli chromosome under different conditions.Nucleic acids research · 2013Article
- Organization and segregation of bacterial chromosomes.Nature reviews. Genetics · 2013Review
- Caulobacter chromosome in vivo configuration matches model predictions for a supercoiled polymer in a cell-like confinement.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Molecular basis for a protein-mediated DNA-bridging mechanism that functions in condensation of the E. coli chromosome.Molecular cell · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The bacterial chromosome is organized into multiple independent domains, each capable of constraining the plectonemic negative supercoil energy introduced by DNA gyrase. Different experimental approaches have estimated the number of domains to be between 40 and 150. The site-specific resolution systems of closely related transposons Tn3 and gammadelta are valuable tools for measuring supercoil diffusion and analysing bacterial chromosome dynamics in vivo. Once made, the wild-type resolvase persists in cells for time periods greater than the cell doubling time. To examine chromosome dynamics over shorter time frames that are more closely tuned to processes like inducible transcription, we constructed a set of resolvases with cellular half-lives ranging from less than 5 min to 30 min. Analysing chromosomes on different time scales shows domain structure to be dynamic. Rather than the 150 domains detected with the Tn3 resolvase, wild-type cells measured over a 10 min time span have more than 400 domains per genome equivalent, and some gyrase mutants exceed 1000.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.