ArticleThe Journal of cell biology2005
A developmentally regulated Na-H exchanger in Dictyostelium discoideum is necessary for cell polarity during chemotaxis.
Article in The Journal of cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- The effects of pH on cortex and condensate formation.The Journal of cell biology · 2026Article
- Electrodiffusion active pump model with asymmetric immersed chemical potentials.bioRxiv : the preprint server for biology · 2026Article
- Intracellular pH dynamics promotes zebrafish larval tail regeneration.bioRxiv : the preprint server for biology · 2026Article
- Nhe1 is required for directional sensing in vegetativeCell adhesion & migration · 2025Article
- Ion Signaling in Cell Motility and Development inBiomolecules · 2024Review
- Intracellular pH Regulates Cancer and Stem Cell Behaviors: A Protein Dynamics Perspective.Frontiers in oncology · 2020Review
- Sodium Transporters in Human Health and Disease.Frontiers in physiology · 2020Review
- KFrontiers in pharmacology · 2020Article
- The physiological regulation of macropinocytosis duringJournal of cell science · 2018Article
- Increased H⁺ efflux is sufficient to induce dysplasia and necessary for viability with oncogene expression.eLife · 2015Article
- The Nck-interacting kinase NIK increases Arp2/3 complex activity by phosphorylating the Arp2 subunit.The Journal of cell biology · 2015Article
- Na⁺/H⁺ exchangers and intracellular pH in perinatal brain injury.Translational stroke research · 2014Review
- Chaperone activation by unfolding.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- PDGFRα signaling in the primary cilium regulates NHE1-dependent fibroblast migration via coordinated differential activity of MEK1/2-ERK1/2-p90RSK and AKT signaling pathways.Journal of cell science · 2013Article
- Article
- The IplA Ca2+ channel of Dictyostelium discoideum is necessary for chemotaxis mediated through Ca2+, but not through cAMP, and has a fundamental role in natural aggregation.Journal of cell science · 2012Article
- Dysregulated pH: a perfect storm for cancer progression.Nature reviews. Cancer · 2011Review
- Nhe1 is essential for potassium but not calcium facilitation of cell motility and the monovalent cation requirement for chemotactic orientation in Dictyostelium discoideum.Eukaryotic cell · 2011Article
- Pollen tubes lacking a pair of K+ transporters fail to target ovules in Arabidopsis.The Plant cell · 2011Article
- Tyrosine phosphorylation-mediated signaling pathways in dictyostelium.Journal of signal transduction · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Increased intracellular H(+) efflux is speculated to be an evolutionarily conserved mechanism necessary for rapid assembly of cytoskeletal filaments and for morphological polarity during cell motility. In Dictyostelium discoideum, increased intracellular pH through undefined transport mechanisms plays a key role in directed cell movement. We report that a developmentally regulated Na-H exchanger in Dictyostelium discoideum (DdNHE1) localizes to the leading edge of polarized cells and is necessary for intracellular pH homeostasis and for efficient chemotaxis. Starved DdNHE1-null cells (Ddnhe1(-)) differentiate, and in response to the chemoattractant cAMP they retain directional sensing; however, they cannot attain a polarized morphology, but instead extend mislocalized pseudopodia around the cell and exhibit decreased velocity. Consistent with impaired polarity, in response to chemoattractant, Ddnhe1(-) cells lack a leading edge localization of F-actin and have significantly attenuated de novo F-actin polymerization but increased abundance of membrane-associated phosphatidylinositol 3,4,5-trisphosphate (PI((3,4,5))P(3)). These findings indicate that during chemotaxis DdNHE1 is necessary for establishing the kinetics of actin polymerization and PI((3,4,5))P(3) production and for attaining a polarized phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.