ArticleThe Journal of experimental medicine2005
The 3' end of the heavy chain constant region locus enhances germline transcription and switch recombination of the four gamma genes.
Article in The Journal of experimental medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Matters of life and death: How estrogen and estrogen receptor binding to the immunoglobulin heavy chain locus may influence outcomes of infection, allergy, and autoimmune disease.Cellular immunology · 2019Review
- Current insights into the mechanism of mammalian immunoglobulin class switch recombination.Critical reviews in biochemistry and molecular biology · 2019Review
- CCCTC-Binding Factor Locks Premature IgH Germline Transcription and Restrains Class Switch Recombination.Frontiers in immunology · 2017Article
- Class-Switch Recombination in the Absence of the IgH 3' Regulatory Region.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Transcription factor Ebf1 regulates differentiation stage-specific signaling, proliferation, and survival of B cells.Genes & development · 2012Article
- Germline deletion of Igh 3' regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific regulation, rearrangement, and insulation of the Igh locus.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Enhancement of antibody class-switch recombination by the cumulative activity of four separate elements.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Dynamic changes in binding of immunoglobulin heavy chain 3' regulatory region to protein factors during class switching.The Journal of biological chemistry · 2011Article
- Homologous elements hs3a and hs3b in the 3' regulatory region of the murine immunoglobulin heavy chain (Igh) locus are both dispensable for class-switch recombination.The Journal of biological chemistry · 2011Article
- Direct interactions of OCA-B and TFII-I regulate immunoglobulin heavy-chain gene transcription by facilitating enhancer-promoter communication.Molecular cell · 2011Article
- Role of PTIP in class switch recombination and long-range chromatin interactions at the immunoglobulin heavy chain locus.Molecular and cellular biology · 2011Article
- Comparison of identical and functional Igh alleles reveals a nonessential role for Eμ in somatic hypermutation and class-switch recombination.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Switch recombination and somatic hypermutation are controlled by the heavy chain 3' enhancer region.The Journal of experimental medicine · 2009Article
- Generating transgenic mice from bacterial artificial chromosomes: transgenesis efficiency, integration and expression outcomes.Transgenic research · 2009Article
- Article
- Replacement of Igamma3 germ-line promoter by Igamma1 inhibits class-switch recombination to IgG3.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- In a model of immunoglobulin heavy-chain (IGH)/MYC translocation, the Igh 3' regulatory region induces MYC expression at the immature stage of B cell development.Genes, chromosomes & cancer · 2007Article
- BAC to immunology--bacterial artificial chromosome-mediated transgenesis for targeting of immune cells.Immunology · 2007Review
- Transcription of a productively rearranged Ig VDJC alpha does not require the presence of HS4 in the IgH 3' regulatory region.Journal of immunology (Baltimore, Md. : 1950) · 2007Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The switch in immunoglobulin (Ig) heavy chain class is preceded by germline transcription and then mediated by a DNA recombination event. To study germline transcription and class switch recombination we used transgenic mice with a 230-kilobase bacterial artificial chromosome that included a rearranged VDJ gene and the entire heavy chain constant region locus. In addition to several lines with intact transgenes, we identified two lines in which the heavy chain locus transgene lacked Calpha and everything 3' of it, including the regulatory elements HS3a, HS1-2, HS3b, and HS4. B cells from both lines with the truncated transgenes make abundant transgenic (Tg) VDJCmu transcripts and IgM protein. Deletion of the 3' end of the locus results in dramatically reduced expression of both germline transcripts and switched VDJCH transcripts of the gamma3, gamma2b, gamma2a, and epsilon genes. In addition, the transgenes lacking the 3' end of the locus express reduced amounts of gamma1 germline transcripts and 2-3% of the amount of Tg IgG1 in tissue culture compared with intact transgenes. Finally, switch recombination to gamma1 is undetectable in the transgenes lacking the 3' elements, as measured by digestion circularization-polymerase chain reaction or by the expression of VDJCgamma1 transcripts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.