ArticleProceedings of the National Academy of Sciences of the United States of America2005
Functional analysis of the pseudoknot structure in human telomerase RNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
66 citing papers in PubMed, 130 citations in OpenAlex.
- Decoding RNA triple helices: identification from sequence and secondary structure.Briefings in bioinformatics · 2026Article
- A degenerate telomerase RNA directs telomeric DNA synthesis in lepidopteran insects.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Recent progress in human telomerase structure and its therapeutic targeting.Frontiers in molecular biosciences · 2025Review
- RNA secondary structure prediction by conducting multi-class classifications.Computational and structural biotechnology journal · 2025Article
- Evaluating Performance of Different RNA Secondary Structure Prediction Programs Using Self-cleaving Ribozymes.Genomics, proteomics & bioinformatics · 2024Article
- Advances in understanding telomerase assembly.Biochemical Society transactions · 2023Review
- Telomerase RNA in Hymenoptera (Insecta) switched to plant/ciliate-like biogenesis.Nucleic acids research · 2023Article
- Article
- Biogenesis of telomerase RNA from a protein-coding mRNA precursor.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- How Structural Features Define Biogenesis and Function of Human Telomerase RNA Primary Transcript.Biomedicines · 2022Review
- Development of RNA G-quadruplex (rG4)-targeting L-RNA aptamers by rG4-SELEX.Nature protocols · 2022Review
- Thermodynamics of unfolding mechanisms of mouse mammary tumor virus pseudoknot from a coarse-grained loop-entropy model.Journal of biological physics · 2022Article
- Evolution of plant telomerase RNAs: farther to the past, deeper to the roots.Nucleic acids research · 2021Article
- Systematics for types and effects of RNA variations.RNA biology · 2021Article
- Monophyletic Origin and Divergent Evolution of Animal Telomerase RNA.Molecular biology and evolution · 2021Article
- RNAs as Regulators of Cellular Matchmaking.Frontiers in molecular biosciences · 2021Review
- The conserved structure of plant telomerase RNA provides the missing link for an evolutionary pathway from ciliates to humans.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Effects of flanking regions on HDV cotranscriptional folding kinetics.RNA (New York, N.Y.) · 2018Article
- New perspectives on telomerase RNA structure and function.Wiley interdisciplinary reviews. RNA · 2018Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Telomerase is essential for maintaining telomere length and chromosome stability in stem cells, germline cells, and cancer cells. The telomerase ribonucleoprotein complex consists of two essential components, a catalytic protein component and an RNA molecule that provides the template for telomeric repeat synthesis. A pseudoknot structure in the human telomerase RNA is conserved in all vertebrates and is essential for telomerase activity. It has been proposed that this highly conserved structure functions as a dynamic structure with conformational interchange between the pseudoknot and a hairpin with intraloop base pairings. To examine the structural and functional requirements of the pseudoknot structure, we made mutations in the proposed base-paired regions in the pseudoknot. Although mutations that disrupted the pseudoknot P3 helix abolished activity as predicted, mutations that disrupted the intraloop hairpin base pairings did not reduce telomerase activity, indicating that the intraloop hairpin is not required for telomerase function. This functional study thus provides evidence against the previous proposed molecular-switch model of telomerase pseudoknot function and supports a static pseudoknot structure. The mutational analysis further suggests that telomerase RNA can function independent of the proposed intermolecular pairings between pseudoknot regions on two RNA molecules.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.