ArticleBiophysical journal2005
Rapid substrate-induced charge movements of the GABA transporter GAT1.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 55 citations in OpenAlex.
- BRAINCELL modelling platform for stochastic nanoscale organisation and dynamic extracellular signalling among neurons and glia.Nature communications · 2026Article
- Volume-transmitted GABA waves pace epileptiform rhythms in the hippocampal network.Current biology : CB · 2023Article
- A comparative review on the well-studied GAT1 and the understudied BGT-1 in the brain.Frontiers in physiology · 2023Review
- Optimizing the Substrate Uptake Rate of Solute Carriers.Frontiers in physiology · 2022Article
- Pre-steady-state Kinetic Analysis of Amino Acid Transporter SLC6A14 Reveals Rapid Turnover Rate and Substrate Translocation.Frontiers in physiology · 2021Article
- Article
- Kinetic Models of Secondary Active Transporters.International journal of molecular sciences · 2019Article
- A comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2.The Journal of general physiology · 2019Article
- Article
- Modeling and Simulation ofComputational and structural biotechnology journal · 2019Article
- Electrogenic Binding of Intracellular Cations Defines a Kinetic Decision Point in the Transport Cycle of the Human Serotonin Transporter.The Journal of biological chemistry · 2016Article
- Evidence for a Revised Ion/Substrate Coupling Stoichiometry of GABA Transporters.The Journal of membrane biology · 2015Article
- Ion transporter NKCC1, modulator of neurogenesis in murine olfactory neurons.The Journal of biological chemistry · 2015Article
- Synaptic GABA release prevents GABA transporter type-1 reversal during excessive network activity.Nature communications · 2015Article
- Sodium-assisted formation of binding and traverse conformations of the substrate in a neurotransmitter sodium symporter model.Current drug discovery technologies · 2014Article
- Structure, function, and plasticity of GABA transporters.Frontiers in cellular neuroscience · 2014Review
- Plasticity of GABA transporters: an unconventional route to shape inhibitory synaptic transmission.Frontiers in cellular neuroscience · 2014Article
- GABA and Glutamate Transporters in Brain.Frontiers in endocrinology · 2013Review
- Functional consequences of sulfhydryl modification of the γ-aminobutyric acid transporter 1 at a single solvent-exposed cysteine residue.The Journal of membrane biology · 2012Article
- Inhibition of Activity of GABA Transporter GAT1 by δ-Opioid Receptor.Evidence-based complementary and alternative medicine : eCAM · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The GABA transporter GAT1 removes the neurotransmitter GABA from the synaptic cleft by coupling of GABA uptake to the co-transport of two sodium ions and one chloride ion. The aim of this work was to investigate the individual reaction steps of GAT1 after a GABA concentration jump. GAT1 was transiently expressed in HEK293 cells and its pre-steady-state kinetics were studied by combining the patch-clamp technique with the laser-pulse photolysis of caged GABA, which allowed us to generate GABA concentration jumps within <100 micros. Recordings of transport currents generated by GAT1, both in forward and exchange transport modes, showed multiple charge movements that can be separated along the time axis. The individual reactions associated with these charge movements differ from the well-characterized electrogenic "sodium-occlusion" reaction by GAT1. One of the observed electrogenic reactions is shown to be associated with the GABA-translocating half-cycle of the transporter, in contradiction to previous studies that showed no charge movements associated with these reactions. Interestingly, reactions of the GABA-bound transporter were not affected by the absence of extracellular chloride, suggesting that Cl- may not be co-translocated with GABA. Based on the results, a new alternating access sequential-binding model is proposed for GAT1's transport cycle that describes the results presented here and those by others.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.