ArticleProceedings of the National Academy of Sciences of the United States of America1992
Proteins on exocytic vesicles mediate calcium-triggered fusion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Applicability of Hyaluronic Acid-Alginate Hydrogel and Ovarian Cells for In Vitro Development of Mouse Preantral Follicles.Cell journal · 2020Article
- Effects of Alginate Concentration and Ovarian Cells on In Vitro Development of Mouse Preantral Follicles: A Factorial Study.International journal of fertility & sterility · 2020Article
- Unraveling the mechanisms of calcium-dependent secretion.The Journal of general physiology · 2019Review
- Application of High-Throughput Assays to Examine Phospho-Modulation of the Late Steps of Regulated Exocytosis.High-throughput · 2017Article
- Quantification of exocytosis kinetics by DIC image analysis of cortical lawns.Journal of chemical biology · 2014Article
- A new approach to the molecular analysis of docking, priming, and regulated membrane fusion.Journal of chemical biology · 2011Article
- Enhancement of the Ca(2+)-triggering steps of native membrane fusion via thiol-reactivity.Journal of chemical biology · 2009Article
- Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.The Journal of physiology · 1999Review
- Biochemical and functional studies of cortical vesicle fusion: the SNARE complex and Ca2+ sensitivity.The Journal of cell biology · 1998Article
- Poisson-distributed active fusion complexes underlie the control of the rate and extent of exocytosis by calcium.The Journal of cell biology · 1996Article
- Annexins in Paramecium cells. Involvement in site-specific positioning of secretory organelles.Histochemistry and cell biology · 1996Article
- Regulatory mechanisms of the acrosome reaction revealed by multiview microscopy of single starfish sperm.The Journal of cell biology · 1995Article
- Application of a membrane fusion assay for rapid drug screening.Pharmaceutical research · 1995Article
- The N-ethylmaleimide-sensitive protein thiol groups necessary for sea-urchin egg cortical-granule exocytosis are highly exposed to the medium and are required for triggering by Ca2+.The Biochemical journal · 1994Article
- Single event recording shows that docking onto receptor alters the kinetics of membrane fusion mediated by influenza hemagglutinin.Biophysical journal · 1993Article
- Focal exocytosis by eosinophils--compound exocytosis and cumulative fusion.The EMBO journal · 1993Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In many exocytic systems, micromolar concentrations of intracellular Ca2+ trigger fusion. We find that aggregates of secretory granules isolated from sea urchin eggs fuse together when perfused with greater than or equal to 10 microM free Ca2+. Mixing of membrane components was demonstrated by transfer of fluorescent lipophilic dye, and melding of granule contents was seen with differential interference microscopy. A technique based upon light scattering was developed to conveniently detect fusion. Two protein modifiers, trypsin and N-ethylmaleimide, inhibit granule-granule fusion at concentrations similar to those that inhibit granule-plasma membrane fusion. We suggest that molecular machinery sufficient for Ca(2+)-triggered fusion resides on secretory granules as purified and that at least some of these essential components are proteinaceous.
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