ArticleJournal of virological methods2005
The use of endogenous and exogenous reference RNAs for qualitative and quantitative detection of PRRSV in porcine semen.
Article in Journal of virological methods, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Safety and efficacy of a novel European vaccine for porcine reproductive and respiratory virus in bred gilts.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Trial
- Safety and early onset of immunity with a novel European porcine reproductive and respiratory syndrome virus vaccine in young piglets.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2016Trial
- Effect of Pooling and Freeze-Thaw Processes on the PRRSV RNA Detection in TOSc Samples.Veterinary sciences · 2025Article
- Assessing recovery rates of distinct exogenous controls for gDNA extraction efficiency using phenol-chloroform or silica-column based extractions.Journal of microbiological methods · 2022Article
- Swine Norovirus: Past, Present, and Future.Viruses · 2022Review
- Article
- RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms.Scientific reports · 2019Article
- Field evaluation of the safety and compatibility of a combined vaccine against porcine parvovirus 1 and porcine reproductive and respiratory syndrome virus in breeding animals.Porcine health management · 2019Article
- Long duration of immunity against a type 1 heterologous PRRS virus challenge in pigs immunised with a novel PRRS MLV vaccine: a randomised controlled study.Porcine health management · 2018Article
- Detection and molecular characterization of porcine reproductive and respiratory syndrome virus in Lithuanian wild boar populations.Acta veterinaria Scandinavica · 2016Article
- Evaluation of Different DNA Vaccines against Porcine Reproductive and Respiratory Syndrome (PRRS) in Pigs.Vaccines · 2013Article
- Comparison of viremia of type II porcine reproductive and respiratory syndrome virus in naturally infected pigs by zip nucleic acid probe-based real-time PCR.BMC veterinary research · 2013Article
- Effects on boar semen quality after infection with porcine reproductive and respiratory syndrome virus: a case report.Acta veterinaria Scandinavica · 2013Article
- Exogenous reference gene normalization for real-time reverse transcription-polymerase chain reaction analysis under dynamic endogenous transcription.Neural regeneration research · 2012Article
- Detection and quantification of infectious avian influenza A (H5N1) virus in environmental water by using real-time reverse transcription-PCR.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Semen is known to be a route of porcine reproductive and respiratory syndrome virus (PRRSV) transmission. A method was developed for qualitative and quantitative detection of the seminal cell-associated PRRSV RNA in relation to endogenous and exogenous reference RNAs. As endogenous control for one-step real-time reverse transcription (RT)-PCR UBE2D2 mRNA was selected. Particularly for the analysis of persistent infections associated with low copy numbers of PRRSV RNA, UBE2D2 mRNA is an ideal control due to its low expression in seminal cells and its detection in all samples analysed (n = 36). However, the amount of UBE2D2 mRNA in porcine semen varied (up to 106-fold), thus its use is limited to qualitative detection of PRRSV RNA. For quantitation, a synthetic, non-metazoan RNA was added to the RNA isolation reaction at an exact copy number. The photosynthesis gene ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) from Arabidopsis thaliana was used as an exogenous spike. Unexpectedly, PRRSV RNA was detected in a herd of specific pathogen-free (SPF) boars which were tested ELISA-negative for anti-PRRSV antibodies. Therefore, RT-PCR for seminal cell-associated PRRSV is a powerful tool for managing the SPF status during quarantine programs and for routine outbreak investigations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.