ArticleThe Journal of physiology2005
Cytoskeletal regulation of calcium-permeable cation channels in the human syncytiotrophoblast: role of gelsolin.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- A placental model of SARS-CoV-2 infection reveals ACE2-dependent susceptibility and differentiation impairment in syncytiotrophoblasts.Nature cell biology · 2023Article
- Placental ion channels: potential target of chemical exposure.Biology of reproduction · 2023Review
- Investigation of Sperm and Seminal Plasma Candidate MicroRNAs of Bulls with Differing Fertility and In Silico Prediction of miRNA-mRNA Interaction Network of Reproductive Function.Animals : an open access journal from MDPI · 2022Article
- Polycystin-2 (TRPP2) Regulation by Ca(2+) Is Effected and Diversified by Actin-Binding Proteins.Biophysical journal · 2015Article
- Plasma gelsolin protects HIV-1 gp120-induced neuronal injury via voltage-gated K+ channel Kv2.1.Molecular and cellular neurosciences · 2013Article
- Interaction between Calcium and Actin in Guard Cell and Pollen Signaling Networks.Plants (Basel, Switzerland) · 2013Review
- Polycystins and cellular Ca2+ signaling.Cellular and molecular life sciences : CMLS · 2013Review
- Calcium transport and local pool regulate polycystin-2 (TRPP2) function in human syncytiotrophoblast.Biophysical journal · 2013Article
- Maternal serum proteome changes between the first and third trimester of pregnancy in rural southern Nepal.Placenta · 2012Article
- Article
- The plasma membrane potential and the organization of the actin cytoskeleton of epithelial cells.International journal of cell biology · 2012Article
- Soluble levels of cytosolic tubulin regulate ciliary length control.Molecular biology of the cell · 2011Article
- Function and regulation of TRPP2 at the plasma membrane.American journal of physiology. Renal physiology · 2009Review
- The multimeric structure of polycystin-2 (TRPP2): structural-functional correlates of homo- and hetero-multimers with TRPC1.Human molecular genetics · 2009Article
- Polycystin-2 cation channel function in the human syncytiotrophoblast is regulated by microtubular structures.The Journal of physiology · 2007Article
- Effect of hydro-osmotic pressure on polycystin-2 channel function in the human syncytiotrophoblast.Pflugers Archiv : European journal of physiology · 2005Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human syncytiotrophoblast (hST) is the most apical epithelial barrier that covers the villous tree of the human placenta. An intricate and highly organized network of cytoskeletal structures supports the hST. Recently, polycystin-2 (PC2), a TRP-type nonselective cation channel, was functionally observed in hST, where it may be an important player to Ca2+ transport. Little is known, however, about channel regulation in hST. In this report, the regulatory role of actin dynamics on PC2 channels reconstituted from hST apical membranes was explored. Acute addition of cytochalasin D (CD, 5 microg ml-1) to reconstituted hST apical membranes transiently increased K+ -permeable channel activity. The actin-binding proteins alpha-actinin and gelsolin, as well as PC2, were observed by Western blot and immunofluorescence analyses in hST vesicles. CD treatment of hST vesicles resulted in a re-distribution of actin filaments, in agreement with the effect of CD on K+ channel activity. In contrast, addition of exogenous monomeric actin, but not prepolymerized actin, induced a rapid inhibition of channel function in hST. This inhibition was obliterated by the presence of CD in the medium. The acute (<15 min) CD stimulation of K+ channel activity was mimicked by addition of the actin-severing protein gelsolin in the presence, but not in the absence, of micromolar Ca2+. Ca2+ transport through PC2 triggers a regulatory feedback mechanism, which is based on the severing and re-formation of filamentous actin near the channels. Cytoskeletal structures may thus be relevant to ion transport regulation in the human placenta.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.