ArticleInfection and immunity2005
Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 134 citations in OpenAlex.
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- Taxonomic distribution of SbmA/BacA and BacA-like antimicrobial peptide transporters suggests independent recruitment and convergent evolution in host-microbe interactions.Microbial genomics · 2025Article
- Detecting co-selection through excess linkage disequilibrium in bacterial genomes.NAR genomics and bioinformatics · 2024Article
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- Proteomic profile of naturally released extracellular vesicles secreted from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses.Scientific reports · 2023Article
- The role of AJB35136 and fdtA genes in biofilm formation by avian pathogenic Escherichia coli.BMC veterinary research · 2023Article
- Translocation of non-lytic antimicrobial peptides and bacteria penetrating peptides across the inner membrane of the bacterial envelope.Current genetics · 2022Review
- Identification of novel genes involved in the biofilm formation process of Avian Pathogenic Escherichia coli (APEC).PloS one · 2022Article
- Molecular mechanism of SbmA, a promiscuous transporter exploited by antimicrobial peptides.Science advances · 2021Article
- Comparative Pathogenomics ofInfection and immunity · 2021Article
- Bacteria primed by antimicrobial peptides develop tolerance and persist.PLoS pathogens · 2021Article
- A One Health Perspective for Defining and DecipheringComparative medicine · 2021Review
- The Rcs System inFrontiers in microbiology · 2021Review
- Assessment of the Presence of Resistance Genes Detected from the Environment and Selected Food Products in Benin.Journal of environmental and public health · 2021Article
- Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines.Applied and environmental microbiology · 2020Article
- Identification of ireA, 0007, 0008, and 2235 as TonB-dependent receptors in the avian pathogenic Escherichia coli strain DE205B.Veterinary research · 2020Article
- Validation of Predicted Virulence Factors inToxins · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infections with avian pathogenic Escherichia coli (APEC) cause colibacillosis, an acute and largely systemic disease resulting in significant economic losses in poultry industry worldwide. Although various virulence-associated genes have been identified in APEC, their actual role in pathogenesis is still not fully understood, and, furthermore, certain steps of the infection process have not been related to previously identified factors. Here we describe the application of a signature-tagged transposon mutagenesis (STM) approach to identify critical genes required for APEC infections in vivo. Twenty pools of about 1,800 IMT5155 (O2:H5) mutants were screened in an infection model using 5-week-old chickens, and potentially attenuated mutants were subjected to a secondary screen and in vivo competition assays to confirm their attenuation. A total of 28 genes required for E. coli septicemia in chickens were identified as candidates for further characterization. Among these disrupted genes, six encode proteins involved in biosynthesis of extracellular polysaccharides and lipopolysaccharides; two encode iron transporters that have not been previously characterized in APEC in in vivo studies, and four showed similarity to membrane or periplasmic proteins. In addition, several metabolic enzymes, putative proteins with unknown function, and open reading frames with no similarity to other database entries were identified. This genome-wide analysis has identified both novel and previously known factors potentially involved in pathogenesis of APEC infection.
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