ArticleChromosoma2005
Non-reciprocal chromosomal bridge-induced translocation (BIT) by targeted DNA integration in yeast.
Article in Chromosoma, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Timing of Chromosome DNA Integration throughout the Yeast Cell Cycle.Biomolecules · 2023Article
- Transposase expression, element abundance, element size, and DNA repair determine the mobility and heritability ofFrontiers in cell and developmental biology · 2023Article
- Article
- Post-translocational adaptation drives evolution through genetic selection and transcriptional shift in Saccharomyces cerevisiae.Current genetics · 2017Article
- Bridge-Induced Translocation betweenFrontiers in oncology · 2017Article
- Multiple Antibiotic Resistance Plasmids Allow Scalable, PCR-Mediated DNA Manipulation and Near-Zero Background Cloning.Food technology and biotechnology · 2016Article
- High reactive oxygen species levels are detected at the end of the chronological life span of translocant yeast cells.Molecular genetics and genomics : MGG · 2016Article
- Review
- Bridge-induced chromosome translocation in yeast relies upon a Rad54/Rdh54-dependent, Pol32-independent pathway.PloS one · 2013Article
- Warburg effect and translocation-induced genomic instability: two yeast models for cancer cells.Frontiers in oncology · 2012Article
- Different aneuploidies arise from the same bridge-induced chromosomal translocation event in Saccharomyces cerevisiae.Genetics · 2010Article
- DNA bridging of yeast chromosomes VIII leads to near-reciprocal translocation and loss of heterozygosity with minor cellular defects.Chromosoma · 2009Article
- Cellular and molecular effects of nonreciprocal chromosome translocations in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Retrosequence formation restructures the yeast genome.Genes & development · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several experimental in vivo systems exist that generate reciprocal translocations between engineered chromosomal loci of yeast or Drosophila, but not without previous genome modifications. Here we report the successful induction of chromosome translocations in unmodified yeast cells via targeted DNA integration of the KAN(R) selectable marker flanked by sequences homologous to two chromosomal loci randomly chosen on the genome. Using this bridge-induced translocation system, 2% of the integrants showed targeted translocations between chromosomes V-VIII and VIII-XV in two wild-type Saccharomyces cerevisiae strains. All the translocation events studied were found to be non-reciprocal and the fate of their chromosomal fragments that were not included in the translocated chromosome was followed. The recovery of discrete-sized fragments suggested multiple pathway repair of their free DNA ends. We propose that centromere-distal chromosome fragments may be processed by a break-induced replication mechanism ensuing in partial trisomy. The experimental feasibility of inducing chromosomal translocations between any two desired genetic loci in a eukaryotic model system will be instrumental in elucidating the molecular mechanism underlying genome rearrangements generated by DNA integration and the gross chromosomal rearrangements characteristic of many types of cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.