ArticlePlant cell reports2005
Transformation of radish (Raphanus sativus L.) via sonication and vacuum infiltration of germinated seeds with Agrobacterium harboring a group 3 LEA gene from B. napus.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Agrobacterium-mediated genetic transformation of chia (Salvia hispanica L.), a rich source of omega-3 fatty acid.Transgenic research · 2025Article
- Overexpression ofHeliyon · 2024Article
- EfficientPlant biotechnology (Tokyo, Japan) · 2021Article
- Late Embryogenesis Abundant Protein-Client Protein Interactions.Plants (Basel, Switzerland) · 2020Review
- Genetic Transformation of Oat Mediated byIranian journal of biotechnology · 2019Article
- Genotype-independent and enhanced in planta3 Biotech · 2018Article
- In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.PloS one · 2016Article
- Article
- An efficient in planta transformation of Jatropha curcas (L.) and multiplication of transformed plants through in vivo grafting.Protoplasma · 2014Article
- Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane.Plant cell reports · 2013Article
- Development of efficient Catharanthus roseus regeneration and transformation system using agrobacterium tumefaciens and hypocotyls as explants.BMC biotechnology · 2012Article
- Improved Agrobacterium-mediated transformation of cowpea via sonication and vacuum infiltration.Plant cell reports · 2011Article
- Genetic engineering of radish: current achievements and future goals.Plant cell reports · 2011Review
- Highly efficient Agrobacterium-mediated transformation of banana cv. Rasthali (AAB) via sonication and vacuum infiltration.Plant cell reports · 2011Article
- Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors.Physiology and molecular biology of plants : an international journal of functional plant biology · 2010Article
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Authors and funding
4 authors.
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Abstract
A protocol for producing transgenic radish (Raphanus sativus) was obtained by using both ultrasonic and vacuum infiltration assisted, Agrobacterium-mediated transformation. The Agrobacterium strain LBA4404 contained the binary vector pBI121-LEA (late embyogenesis abundant), which carried a Group 3 LEA gene, from Brassica napus. Among six combinations, Agrobacterium-mediated transformation assisted by a combination of 5-min sonication with 5-min vacuum infiltration resulted in the highest transformation frequency. The existence, integration and expression of transferred LEA gene in transgenic T(1) plants were confirmed by PCR, genomic Southern and Western blot analysis. Transgenic radish demonstrated better growth performance than non-transformed control plants under osmotic and salt stress conditions. Accumulation of Group 3 LEA protein in the vegetative tissue of transgenic radish conferred increased tolerance to water deficit and salt stress.
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