ArticleNucleic acids research2005
Cryptic promoter activity in the DNA sequence corresponding to the pim-1 5'-UTR.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 51 citations in OpenAlex.
- When RNA goes off script: ensuring transcript fidelity in transgene expression.The EMBO journal · 2026Review
- Evaluating the reliability of tools for mRNA annotation and IRES studies.bioRxiv : the preprint server for biology · 2026Article
- MYC upstream region orchestrates resistance to PI3K inhibitors in cancer cells through FOXO3a-mediated autophagic adaptation.Oncogene · 2024Article
- Internal ribosomal entry site-mediated translational activity of nitric oxide synthase 2.Animal cells and systems · 2023Article
- Revisiting the pathogenic mechanism of the GJB1 5' UTR c.-103C > T mutation causing CMTX1.Neurogenetics · 2021Article
- PTBP1 Positively Regulates the Translation of Circadian Clock Gene,International journal of molecular sciences · 2020Article
- hnRNP Q Regulates Internal Ribosome Entry Site-MediatedMolecular and cellular biology · 2019Article
- Screening for functional IRESes using α-complementation system of β-galactosidase inBiotechnology for biofuels · 2019Article
- Identification of Rhopalosiphum Padi Virus 5' Untranslated Region Sequences Required for Cryptic Promoter Activity and Internal Ribosome Entry.International journal of molecular sciences · 2015Article
- PIM kinase (and Akt) biology and signaling in tumors.Pharmacology & therapeutics · 2015Review
- The current status of vertebrate cellular mRNA IRESs.Cold Spring Harbor perspectives in biology · 2013Review
- Conditional transgenic expression of PIM1 kinase in prostate induces inflammation-dependent neoplasia.PloS one · 2013Article
- Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function.Nucleic acids research · 2012Article
- Rhythmic interaction between Period1 mRNA and hnRNP Q leads to circadian time-dependent translation.Molecular and cellular biology · 2012Article
- Alternative ways to think about cellular internal ribosome entry.The Journal of biological chemistry · 2010Review
- Evidence for translational regulation by the herpes simplex virus virion host shutoff protein.Journal of virology · 2010Article
- Autoregulation of co-chaperone BAG3 gene transcription.Journal of cellular biochemistry · 2009Article
- The XIAP IRES activates 3' cistron expression by inducing production of monocistronic mRNA in the betagal/CAT bicistronic reporter system.RNA (New York, N.Y.) · 2009Article
- Differential contribution of the m7G-cap to the 5' end-dependent translation initiation of mammalian mRNAs.Nucleic acids research · 2009Article
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Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The serine/threonine kinase pim-1 mRNA contains a long and G/C rich 5'-untranslated region (5'-UTR). Previous work suggested that the pim-1 5'-UTR harbors an internal ribosomal entry site (IRES) allowing for internal initiation of translation. However, several previously reported eukaryotic IRES elements actually contain cryptic promoter activity. To test whether an IRES or a cryptic promoter is present in the pim-1 5'-UTR, the 5'-UTR was re-examined using stringent test procedures. Our results show the presence of strong promoter activity in the DNA sequence corresponding to the pim-1 5'-UTR. Both promoterless dicistronic test and northern blot analysis show transcripts being derived from the cryptic promoter in the pim-1 5'-UTR sequence. This cryptic promoter is active in all cell types tested, including Cos-7, NIH3T3, HEK293, Jurkat and K562 cells. When a dicistronic mRNA containing the pim-1 5'-UTR was translated in vitro or in vivo, no IRES activity could be detected. However, the control IRESs from both human rhinovirus and encephalomyocarditis virus exhibited strong IRES activities. In addition, both the RNase protection assay and the 5'-RACE assay detected endogenous pim-1 transcripts with shorter 5'-UTRs. Our data strongly suggest that the IRES activity reported earlier for the pim-1 5'-UTR sequence is due to cryptic promoter activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.