ArticleNucleic acids research2005
Pre-mRNA processing enhancer (PPE) elements from intronless genes play additional roles in mRNA biogenesis than do ones from intron-containing genes.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Coordinating mRNA maturation: The U1 relay model.Molecular cell · 2026Review
- Sequence Determinants for Nuclear Retention and Cytoplasmic Export of mRNAs and lncRNAs.Frontiers in genetics · 2018Review
- Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.Nucleic acids research · 2013Article
- Crosstalk between Wnt Signaling and RNA Processing in Colorectal Cancer.Journal of Cancer · 2013Article
- Export and stability of naturally intronless mRNAs require specific coding region sequences and the TREX mRNA export complex.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- The presence of multiple introns is essential for ERECTA expression in Arabidopsis.RNA (New York, N.Y.) · 2011Article
- Cryptic splice sites and split genes.Nucleic acids research · 2011Article
- Identification of an effective siRNA target site and functional regulatory elements, within the hepatitis B virus posttranscriptional regulatory element.Virology journal · 2010Article
- A functional human Poly(A) site requires only a potent DSE and an A-rich upstream sequence.The EMBO journal · 2010Article
- Posttranscriptional regulatory elements enhance antigen expression and DNA vaccine efficacy.DNA and cell biology · 2009Article
- Activation of pattern recognition receptor-mediated innate immunity inhibits the replication of hepatitis B virus in human hepatocyte-derived cells.Journal of virology · 2009Article
- Where do introns come from?PLoS biology · 2008Article
- Two G-rich regulatory elements located adjacent to and 440 nucleotides downstream of the core poly(A) site of the intronless melanocortin receptor 1 gene are critical for efficient 3' end processing.Molecular and cellular biology · 2007Article
- The hepatitis B virus PRE contains a splicing regulatory element.Nucleic acids research · 2006Article
- Binding of hnRNP L to the pre-mRNA processing enhancer of the herpes simplex virus thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Most mRNA-encoding genes require introns for efficient expression in high eukaryotes. However, mRNAs can efficiently accumulate in the cytoplasm without intron excision if they contain cis-acting elements such as the post-transcriptional regulatory element (PRE) of hepatitis B virus (HBV), the constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV), or the pre-mRNA processing enhancer (PPE) of herpes simplex virus' thymidine kinase (HSV-TK) gene. We compared the activities of these viral elements, the Rev-responsive element (RRE) of the human immunodeficiency virus (HIV), and the human c-Jun gene's enhancer (CJE), an element newly identified here, to enable expression of an intronless variant of the human beta-globin gene. The PRE, PPE and CJE from naturally intronless genes, but not the CTE or RRE from intron-containing genes, significantly enhanced stability, 3' end processing and cytoplasmic accumulation. When the transcripts included the beta-globin gene's first intron, the PRE, PPE and CJE still enhanced mRNA biogenesis, in some cases without intron excision. Thus, elements enabling stability, 3' end formation and nucleocytoplasmic export, not the presence of introns or their excision per se, are necessary for mRNA biogenesis. While the CTE and RRE primarily enhance nucleocytoplasmic export, PPE-like elements from naturally intronless genes facilitate polyadenylation as well.
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