ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Use of laser-capture microdissection for the identification of marker genes for the ventromedial hypothalamic nucleus.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 107 citations in OpenAlex.
- On the Pleiotropic Actions of Glucagon-like Peptide-1 in Its Regulation of Homeostatic and Hedonic Feeding.International journal of molecular sciences · 2025Article
- Canonical transient receptor potential channels and hypothalamic control of homeostatic functions.Journal of neuroendocrinology · 2024Review
- Ventromedial hypothalamic OGT drives adipose tissue lipolysis and curbs obesity.Science advances · 2022Article
- The PACAP Paradox: Dynamic and Surprisingly Pleiotropic Actions in the Central Regulation of Energy Homeostasis.Frontiers in endocrinology · 2022Review
- Adaptive Changes in the Central Control of Energy Homeostasis Occur in Response to Variations in Energy Status.International journal of molecular sciences · 2021Review
- Electroconvulsive seizures lead to lipolytic-induced gene expression changes in mediobasal hypothalamus and decreased white adipose tissue mass.Neuropsychopharmacology reports · 2021Article
- A fish with no sex: gonadal and adrenal functions partition between zebrafish NR5A1 co-orthologs.Genetics · 2021Article
- Deletion of growth hormone receptor in hypothalamic neurons affects the adaptation capacity to aerobic exercise.Peptides · 2021Article
- Coordination of Multiple Cellular Processes by NR5A1/Nr5a1.Endocrinology and metabolism (Seoul, Korea) · 2020Review
- Integrative analysis of DNA methylation-driven genes for the prognosis of lung squamous cell carcinoma using MethylMix.International journal of medical sciences · 2020Article
- MicroRNA-7a overexpression in VMH restores the sympathoadrenal response to hypoglycemia.JCI insight · 2019Article
- Distinct temporal requirements for Sonic hedgehog signaling in development of the tuberal hypothalamus.Development (Cambridge, England) · 2018Article
- Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees.Advances in neurobiology · 2018Article
- Endocannabinoid Signaling at Hypothalamic Steroidogenic Factor-1/Proopiomelanocortin Synapses Is Sex- and Diet-Sensitive.Frontiers in molecular neuroscience · 2018Article
- Hypothalamic AMPK-ER Stress-JNK1 Axis Mediates the Central Actions of Thyroid Hormones on Energy Balance.Cell metabolism · 2017Article
- Global analysis of gene expression mediated by OX1 orexin receptor signaling in a hypothalamic cell line.PloS one · 2017Article
- Parvalbumin-Neurons of the Ventrolateral Hypothalamic Parvafox Nucleus Receive a Glycinergic Input: A Gene-Microarray Study.Frontiers in molecular neuroscience · 2017Article
- PACAP intraperitoneal treatment suppresses appetite and food intake via PAC1 receptor in mice by inhibiting ghrelin and increasing GLP-1 and leptin.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Deficiency of Prdm13, a dorsomedial hypothalamus-enriched gene, mimics age-associated changes in sleep quality and adiposity.Aging cell · 2015Article
- Regionalized differentiation of CRH, TRH, and GHRH peptidergic neurons in the mouse hypothalamus.Brain structure & function · 2014Article
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Authors and funding
12 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ventromedial hypothalamic nucleus (VMH) plays an important role in the control of feeding and energy homeostasis. In contrast to other hypothalamic nuclei that are also known to regulate energy balance, there is a paucity of nucleus-specific marker genes for the VMH, limiting the application of molecular approaches for analyzing VMH information processing, function, and circuitry. Here, we report the use of laser-capture microdissection to isolate a set of cDNAs that are enriched in the VMH relative to two adjacent hypothalamic nuclei, the arcuate and dorsomedial hypothalamus. The relative expression levels of nine of the 12 most robustly expressed VMH-enriched genes were confirmed by real-time PCR analysis using separate RNAs from these three nuclei. Three of these VMH-enriched genes were further characterized by in situ hybridization histochemistry, including pituitary adenylate cyclase activating polypeptide, cerebellin 1, and an expressed sequence tag named LBH2. Finally, to test whether some of these genes were coordinately regulated, we monitored their expression in steroidogenic factor 1 (SF-1) knock-out mice. SF-1 is a transcription factor that controls the development of the VMH. The RNA levels for four of these genes were reduced in these knock-out animals, further suggesting that they are direct or indirect targets of this orphan nuclear receptor. The VMH-enriched genes identified here provide a basis for a functional analysis of VMH neuronal subpopulations via the use of bacterial artificial chromosome transgenics and related technologies. These results also demonstrate the utility of laser-capture microdissection coupled with microarray technology to identify nucleus-specific transcriptional networks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.