ArticleCellular microbiology2005
Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells.
Article in Cellular microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 57 citations in OpenAlex.
- Temperature Affects the Host Range of Rhabdochlamydia porcellionis.Applied and environmental microbiology · 2023Article
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- Functions of Sphingolipids in Pathogenesis During Host-Pathogen Interactions.Frontiers in microbiology · 2021Review
- A M35 family metalloprotease is required for fungal virulence against insects by inactivating host prophenoloxidases and beyond.Virulence · 2020Article
- Eukaryotic Cell Permeabilisation to Identify New Putative Chlamydial Type III Secretion System Effectors Secreted within Host Cell Cytoplasm.Microorganisms · 2020Article
- A predation assay using amoebae to screen for virulence factors unearthed the first W. chondrophila inclusion membrane protein.Scientific reports · 2019Article
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- Bactrocera dorsalis male sterilization by targeted RNA interference of spermatogenesis: empowering sterile insect technique programs.Scientific reports · 2016Article
- A phenotype at last: essential role for the Yersinia enterocolitica Ysa type III secretion system in a Drosophila melanogaster S2 cell model.Infection and immunity · 2013Article
- Drosophila Schneider 2 (S2) cells: a novel tool for studying HSV-induced membrane fusion.Virology · 2013Article
- Identification of critical host mitochondrion-associated genes during Ehrlichia chaffeensis infections.Infection and immunity · 2012Article
- Lack of effective anti-apoptotic activities restricts growth of Parachlamydiaceae in insect cells.PloS one · 2012Article
- Identification of Drosophila gene products required for phagocytosis of Leishmania donovani.PloS one · 2012Article
- The Drosophila melanogaster host model.Journal of oral microbiology · 2012Article
- Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors.The Journal of cell biology · 2011Article
- How the insect immune system interacts with an obligate symbiotic bacterium.Proceedings. Biological sciences · 2011Article
- Porphyromonas gingivalis-host interactions in a Drosophila melanogaster model.Infection and immunity · 2011Article
- Rab6 and Rab11 regulate Chlamydia trachomatis development and golgin-84-dependent Golgi fragmentation.PLoS pathogens · 2009Article
- Drosophila embryos as model systems for monitoring bacterial infection in real time.PLoS pathogens · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chlamydia spp. are major causes of important human diseases, but dissecting the host-pathogen interactions has been hampered by the lack of bacterial genetics and the difficulty in carrying out forward genetic screens in mammalian hosts. RNA interference (RNAi)-based methodologies for gene inactivation can now be easily carried out in genetically tractable model hosts, such as Drosophila melanogaster, and offer a new approach to identifying host genes required for pathogenesis. We tested whether Chlamydia trachomatis infection of D. melanogaster S2 cells recapitulated critical aspects of mammalian cell infections. As in mammalian cells, C. trachomatis entry was greatly reduced by heparin and cytochalasin D. Inclusions were formed in S2 cells, acquired Golgi-derived sphingolipids, and avoided phagolysosomal fusion. Elementary body (EB) to reticulate body (RB) differentiation was observed, however, no RB to EB development or host cell killing was observed. RNAi-mediated inactivation of Rac, a Rho GTPase recently shown to be required for C. trachomatis entry in mammalian cells, inhibits C. trachomatis infection in S2 cells. We conclude that Drosophila S2 cells faithfully mimic early events in Chlamydia host cell interactions and provides a bona fide system to systematically dissect host functions important in the pathogenesis of obligate intracellular pathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.