ArticleJournal of bacteriology2005
Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
57 citing papers in PubMed, 98 citations in OpenAlex.
- Creating a Library of Random Transposon Mutants in Leptospira.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The Single-Step Method of RNA Purification Applied to Leptospira.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Transposon Sequencing in Leptospira interrogans.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Bioinformatic Identification of CRISPR-Cas Systems inPathogens (Basel, Switzerland) · 2025Article
- Leptospiral adhesins: from identification to future perspectives.Frontiers in microbiology · 2024Review
- Hematogenous dissemination of pathogenic and non-pathogenicFrontiers in cellular and infection microbiology · 2022Article
- Anti-Leptospira immunoglobulin profiling in mice reveals strain specific IgG and persistent IgM responses associated with virulence and renal colonization.PLoS neglected tropical diseases · 2021Article
- Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37 °C.Scientific reports · 2021Article
- The transcriptional response of pathogenic Leptospira to peroxide reveals new defenses against infection-related oxidative stress.PLoS pathogens · 2020Article
- Heterologous Expression of the Pathogen-Specific LIC11711 Gene in the SaprophytePathogens (Basel, Switzerland) · 2020Article
- The zoonotic pathogen Leptospira interrogans mitigates environmental stress through cyclic-di-GMP-controlled biofilm production.NPJ biofilms and microbiomes · 2020Article
- Escape of TLR5 Recognition byFrontiers in immunology · 2020Article
- Creating a Library of Random Transposon Mutants in Leptospira.Methods in molecular biology (Clifton, N.J.) · 2020Article
- PathogenicOpen veterinary journal · 2018Review
- Lvr, a Signaling System That Controls Global Gene Regulation and Virulence in PathogenicFrontiers in cellular and infection microbiology · 2018Article
- LipL21 lipoprotein binding to peptidoglycan enables Leptospira interrogans to escape NOD1 and NOD2 recognition.PLoS pathogens · 2017Article
- The EbpA-RpoN Regulatory Pathway of the Pathogen Leptospira interrogans Is Essential for Survival in the Environment.Applied and environmental microbiology · 2017Article
- Animal Models of Leptospirosis: Of Mice and Hamsters.Frontiers in immunology · 2017Review
- Adipose tissue is the first colonization site of Leptospira interrogans in subcutaneously infected hamsters.PloS one · 2017Article
- Whole Genome Shotgun Sequencing Shows Selection on Leptospira Regulatory Proteins During in vitro Culture Attenuation.The American journal of tropical medicine and hygiene · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent availability of the complete genome sequences of Leptospira interrogans, the agent of leptospirosis, has allowed the identification of several putative virulence factors. However, to our knowledge, attempts to carry out gene transfer in pathogenic Leptospira spp. have failed so far. In this study, we show that the Himar1 mariner transposon permits random mutagenesis in the pathogen L. interrogans. We have identified genes that have been interrupted by Himar1 insertion in 35 L. interrogans mutants. This approach of transposon mutagenesis will be useful for understanding the spirochetal physiology and the pathogenic mechanisms of Leptospira, which remain largely unknown.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.