ArticleJournal of bacteriology2005
Isolation and characterization of FecA- and FeoB-mediated iron acquisition systems of the spirochete Leptospira biflexa by random insertional mutagenesis.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 79 citations in OpenAlex.
- Transposon Sequencing in Leptospira interrogans.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Signal-sensing triggers the shutdown of HemKR, regulating heme and iron metabolism in the spirochete Leptospira biflexa.PloS one · 2024Article
- The fciTABC and feoABI systems contribute to ferric citrate acquisition in Stenotrophomonas maltophilia.Journal of biomedical science · 2022Article
- The FUR-like regulators PerRA and PerRB integrate a complex regulatory network that promotes mammalian host-adaptation and virulence of Leptospira interrogans.PLoS pathogens · 2021Article
- Leptospiral Infection, Pathogenesis and Its Diagnosis-A Review.Pathogens (Basel, Switzerland) · 2021Review
- The sigma factor σMolecular microbiology · 2018Article
- Pneumonia infection in mice reveals the involvement of the feoA gene in the pathogenesis of Acinetobacter baumannii.Virulence · 2018Article
- PathogenicOpen veterinary journal · 2018Review
- Micronutrients and Leptospirosis: A Review of the Current Evidence.PLoS neglected tropical diseases · 2016Review
- Multiple Posttranslational Modifications of Leptospira biflexa Proteins as Revealed by Proteomic Analysis.Applied and environmental microbiology · 2016Article
- Comparative genomic analyses of transport proteins encoded within the genomes of Leptospira species.Microbial pathogenesis · 2015Article
- Structural and functional characterization of an orphan ATP-binding cassette ATPase involved in manganese utilization and tolerance in Leptospira spp.Journal of bacteriology · 2013Article
- Positive regulation of Leptospira interrogans kdp expression by KdpE as Demonstrated with a novel β-galactosidase reporter in Leptospira biflexa.Applied and environmental microbiology · 2012Article
- Transcriptional response of Leptospira interrogans to iron limitation and characterization of a PerR homolog.Infection and immunity · 2010Article
- Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses.Future microbiology · 2010Review
- Leptospira: a spirochaete with a hybrid outer membrane.Molecular microbiology · 2010Review
- Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesis.Genetica · 2010Review
- Review
- Evaluation of the expression and protective potential of Leptospiral sphingomyelinases.Current microbiology · 2010Article
- Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen.Nature reviews. Microbiology · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The specific mechanisms by which Leptospira spp. acquire iron from their ecological niches are unknown. A major factor contributing to our ignorance of spirochetal biology is the lack of methods for genetic analysis of these organisms. In this study, we have developed a system for random transposon mutagenesis of Leptospira biflexa using a mariner transposon, Himar1. To demonstrate the validity of Himar1 in vivo transposon mutagenesis in L. biflexa, a screen of mutants for clones impaired in amino acid biosynthesis was first performed, enabling the identification of tryptophan and glutamate auxotrophs. To investigate iron transporters, 2,000 L. biflexa transposon mutants were screened onto media with and without hemin, thus allowing the identification of five hemin-requiring mutants, and the putative genes responsible for this phenotype were identified. Three mutants had distinct insertions in a gene encoding a protein which shares homology with the TonB-dependent receptor FecA, involved in ferric citrate transport. We also identified two mutants with a Himar1 insertion into a feoB-like gene, the product of which is required for ferrous iron uptake in many bacterial organisms. Interestingly, the growth inhibition exhibited by the fecA and feoB mutants was relieved by deferoxamine, suggesting the presence of a ferric hydroxamate transporter. These results confirm the importance of iron for the growth of Leptospira and its ability to use multiple iron sources.
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