ArticleJournal of bacteriology2005
Two distinct functions of ComW in stabilization and activation of the alternative sigma factor ComX in Streptococcus pneumoniae.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 75 citations in OpenAlex.
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- The SpxA1-TenA toxin-antitoxin system regulates epigenetic variations of Streptococcus pneumoniae by targeting protein synthesis.PLoS pathogens · 2024Article
- Diverse regulatory pathways modulate bet hedging of competence induction in epigenetically-differentiated phase variants of Streptococcus pneumoniae.Nucleic acids research · 2023Article
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- Investigating theMicrobiology (Reading, England) · 2022Article
- Pathogenicity and virulence ofVirulence · 2021Review
- The alternative sigma factor σeLife · 2020Article
- Regulation of pneumococcal epigenetic and colony phases by multiple two-component regulatory systems.PLoS pathogens · 2020Article
- Designing cyclic competence-stimulating peptide (CSP) analogs with pan-group quorum-sensing inhibition activity inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- ComWΔ6 Stimulates Transcription of Pneumococcal Competence GenesFrontiers in molecular biosciences · 2020Article
- Amino acids as nutritional factors and (p)ppGpp as an alarmone of the stringent response regulate natural transformation in Micrococcus luteus.Scientific reports · 2019Article
- The pneumococcal σThe Journal of biological chemistry · 2019Article
- Intensive targeting of regulatory competence genes by transposable elements in streptococci.Molecular genetics and genomics : MGG · 2019Article
- Competence inFrontiers in cellular and infection microbiology · 2019Review
- Dynamic Modeling ofFrontiers in microbiology · 2018Article
- A programmed cell division delay preserves genome integrity during natural genetic transformation in Streptococcus pneumoniae.Nature communications · 2017Article
- Quorum Sensing Regulation of Competence and Bacteriocins in Streptococcus pneumoniae and mutans.Genes · 2017Review
- Competence for Genetic Transformation in Streptococcus pneumoniae: Mutations in σA Bypass the ComW Requirement for Late Gene Expression.Journal of bacteriology · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural genetic transformation in Streptococcus pneumoniae is controlled by a quorum-sensing system, which acts through the competence-stimulating peptide (CSP) for transient activation of genes required for competence. More than 100 genes have been identified as CSP regulated by use of DNA microarray analysis. One of the CSP-induced genes required for genetic competence is comW. As the expression of this gene depended on the regulator ComE, but not on the competence sigma factor ComX (sigma(X)), and as expression of several genes required for DNA processing was affected in a comW mutant, comW appears to be a new regulatory gene. Immunoblotting analysis showed that the amount of the sigma(X) protein is dependent on ComW, suggesting that ComW may be directly or indirectly involved in the accumulation of sigma(X). As sigma(X) is stabilized in clpP mutants, a comW mutation was introduced into the clpP background to ask whether the synthesis of sigma(X) depends on ComW. The clpP comW double mutant accumulated an amount of sigma(X) higher (threefold) than that seen in the wild type but was not transformable, suggesting that while comW is not needed for sigma(X) synthesis, it acts both in stabilization of sigma(X) and in its activation. Modification of ComW with a histidine tag at its C or N terminus revealed that both amino and carboxyl termini are important for increasing the stability of sigma(X), but only the N terminus is important for stimulating its activity.
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