ArticleJournal of bacteriology2005
Characterization of a cluster of three glycosyltransferase enzymes essential for Moraxella catarrhalis lipooligosaccharide assembly.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- A global perspective on the genomics ofMicrobial genomics · 2025Article
- Panel 4: Report of the Microbiology Panel.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2017Review
- Use of next generation sequence to investigate potential novel macrolide resistance mechanisms in a population of Moraxella catarrhalis isolates.Scientific reports · 2016Article
- Flashy flagella: flagellin modification is relatively common and highly versatile among the Enterobacteriaceae.BMC genomics · 2016Article
- An Unusual Carbohydrate Conformation is Evident in Moraxella catarrhalis Oligosaccharides.Molecules (Basel, Switzerland) · 2015Article
- Characterization of a trifunctional glucosyltransferase essential for Moraxella catarrhalis lipooligosaccharide assembly.Glycobiology · 2013Article
- The K1 capsular polysaccharide from Acinetobacter baumannii is a potential therapeutic target via passive immunization.Infection and immunity · 2013Article
- Characterization of the molecular interplay between Moraxella catarrhalis and human respiratory tract epithelial cells.PloS one · 2013Article
- Characterization of the six glycosyltransferases involved in the biosynthesis of Yersinia enterocolitica serotype O:3 lipopolysaccharide outer core.The Journal of biological chemistry · 2010Article
- Use of Moraxella catarrhalis lipooligosaccharide mutants to identify specific oligosaccharide epitopes recognized by human serum antibodies.Infection and immunity · 2009Article
- Comparative proteomic analysis of the Haemophilus ducreyi porin-deficient mutant 35000HP::P2AB.Journal of bacteriology · 2009Article
- Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis.The FEBS journal · 2008Article
- Galactose residues on the lipooligosaccharide of Moraxella catarrhalis 26404 form the epitope recognized by the bactericidal antiserum from conjugate vaccination.Infection and immunity · 2008Article
- Multiplex PCR assay that identifies the major lipooligosaccharide serotype expressed by Moraxella catarrhalis clinical isolates.Journal of clinical microbiology · 2005Article
- Moraxella catarrhalis bacterium without endotoxin, a potential vaccine candidate.Infection and immunity · 2005Article
- Two Glycosyltransferase Genes ofFrontiers in cellular and infection microbiologyArticle
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Moraxella catarrhalis isolates express lipooligosaccharide (LOS) molecules on their surface, which share epitopes similar to that of the Neisseria and Haemophilus species. These common LOS epitopes have been implicated in various steps of pathogenesis for the different organisms. In this study, a cluster of three LOS glycosyltransferase genes (lgt) were identified in M. catarrhalis 7169, a strain that produces a serotype B LOS. Mutants in these glycosyltransferase genes were constructed, and the resulting LOS phenotypes were consistent with varying degrees of truncation compared to wild-type LOS. The LOS structures of each lgt mutant were no longer detected by a monoclonal antibody (MAb 4G5) specific to a highly conserved terminal epitope nor by a monoclonal antibody (MAb 3F7) specific to the serotype B LOS side chain. Mass spectrometry of the LOS glycoforms assembled by two of these lgt mutants indicated that lgt1 encodes an alpha(1-2) glucosyltransferase and the lgt2 encodes a beta(1-4) galactosyltransferase. However, these structural studies could not delineate the function for lgt3. Therefore, M. catarrhalis lgt3 was introduced into a defined beta(1-4) glucosyltransferase Haemophilus ducreyi 35000glu- mutant in trans, and monoclonal antibody analysis confirmed that Lgt3 complemented the LOS defect. These data suggest that lgt3 encodes a glucosyltransferase involved in the addition of a beta(1-4)-linked glucose to the inner core. Furthermore, we conclude that this enzymatic step is essential for the assembly of the complete LOS glycoform expressed by M. catarrhalis 7169.
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