ArticleJournal of bacteriology2005
The DotL protein, a member of the TraG-coupling protein family, is essential for Viability of Legionella pneumophila strain Lp02.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- Review
- Sodium levels and grazing pressure shape natural communities of the intracellular pathogen Legionella.Microbiome · 2023Article
- Recent advances in structural studies of the Legionella pneumophila Dot/Icm type IV secretion system.Microbiology and immunology · 2022Review
- Article
- Evolutionary Dissection of the Dot/Icm System Based on Comparative Genomics of 58 Legionella Species.Genome biology and evolution · 2019Article
- A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel.Nature microbiology · 2018Article
- Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system.Nature communications · 2018Article
- Structural insights into the roles of the IcmS-IcmW complex in the type IVb secretion system ofProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Demonstration that a Klebsiella pneumoniae subsp. pneumoniae isolated from an insect (Nezara viridula) harbors a plasmid-borne type IV secretion system.Current microbiology · 2017Article
- Structural biology: Loading T4SS substrates.Nature microbiology · 2017Article
- Architecture of the type IV coupling protein complex of Legionella pneumophila.Nature microbiology · 2017Article
- ClpP-deletion impairs the virulence of Legionella pneumophila and the optimal translocation of effector proteins.BMC microbiology · 2016Article
- Genetically distinct pathways guide effector export through the type VI secretion system.Molecular microbiology · 2014Article
- Structural organisation of the type IV secretion systems.Current opinion in microbiology · 2014Review
- Poison domains block transit of translocated substrates via the Legionella pneumophila Icm/Dot system.Infection and immunity · 2013Article
- Article
- Phylogenetic reconstruction of the Legionella pneumophila Philadelphia-1 laboratory strains through comparative genomics.PloS one · 2013Article
- The Legionella IcmSW complex directly interacts with DotL to mediate translocation of adaptor-dependent substrates.PLoS pathogens · 2012Article
- Implication of proteins containing tetratricopeptide repeats in conditional virulence phenotypes of Legionella pneumophila.Journal of bacteriology · 2012Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Legionella pneumophila is able to survive inside phagocytic cells by an internalization route that bypasses fusion of the nascent phagosome with the endocytic pathway to allow formation of a replicative phagosome. The dot/icm genes, a major virulence system of L. pneumophila, encode a type IVB secretion system that is required for intracellular growth. One Dot protein, DotL, has sequence similarity to type IV secretion system coupling proteins (T4CPs). In other systems, coupling proteins are not required for viability of the organism. Here we report the first example of a strain, L. pneumophila Lp02, in which a putative T4CP is essential for viability of the organism on bacteriological media. This result is particularly surprising since the majority of the dot/icm genes in Lp02 are dispensable for growth outside of a host cell, a condition that does not require a functional Dot/Icm secretion complex. We were able to isolate suppressors of the Delta dotL lethality and found that many contained mutations in other components of the Dot/Icm secretion system. A systematic analysis of dot/icm deletion mutants revealed that the majority of them (20 of 26) suppressed the lethality phenotype, indicating a partially assembled secretion system may be the source of Delta dotL toxicity in the wild-type strain. These results are consistent with a model in which the DotL protein plays a role in regulating the activity of the L. pneumophila type IV secretion apparatus.
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