ArticleProceedings of the National Academy of Sciences of the United States of America2005
Dual targeting of the protein disulfide isomerase RB60 to the chloroplast and the endoplasmic reticulum.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 98 citations in OpenAlex.
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- The DnaJ-Like Zinc-Finger Protein HCF222 Is Required for Thylakoid Membrane Biogenesis in Plants.Plant physiology · 2017Article
- CCS2, an Octatricopeptide-Repeat Protein, Is Required for Plastid CytochromeFrontiers in plant science · 2017Article
- Selenocystamine improves protein accumulation in chloroplasts of eukaryotic green algae.AMB Express · 2015Article
- Chloroplast localization of Cry1Ac and Cry2A protein--an alternative way of insect control in cotton.Biological research · 2015Article
- Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal.Frontiers in plant science · 2015Review
- The protein disulfide isomerase 1 of Phytophthora parasitica (PpPDI1) is associated with the haustoria-like structures and contributes to plant infection.Frontiers in plant science · 2015Article
- Oxidative stress contributes to autophagy induction in response to endoplasmic reticulum stress in Chlamydomonas reinhardtii.Plant physiology · 2014Article
- Using the SUBcellular database for Arabidopsis proteins to localize the Deg protease family.Frontiers in plant science · 2014Article
- Molecular characterization and expression profiling of the protein disulfide isomerase gene family in Brachypodium distachyon L.PloS one · 2014Article
- The oxidative protein folding machinery in plant cells.Protoplasma · 2013Review
- How protein targeting to primary plastids via the endomembrane system could have evolved? A new hypothesis based on phylogenetic studies.Biology direct · 2013Article
- The dual targeting ability of type II NAD(P)H dehydrogenases arose early in land plant evolution.BMC plant biology · 2013Article
- Oxidative protein-folding systems in plant cells.International journal of cell biology · 2013Review
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- Haematococcus as a promising cell factory to produce recombinant pharmaceutical proteins.Molecular biology reports · 2012Article
- Chlapsin, a chloroplastidial aspartic proteinase from the green algae Chlamydomonas reinhardtii.Planta · 2012Article
- Protein disulfide isomerase-2 of Arabidopsis mediates protein folding and localizes to both the secretory pathway and nucleus, where it interacts with maternal effect embryo arrest factor.Molecules and cells · 2011Article
- The Protein Disulfide Isomerase gene family in bread wheat (T. aestivum L.).BMC plant biology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RB60 is an atypical protein disulfide isomerase (PDI) that functions as a member of a redox regulatory protein complex controlling translation in the chloroplast of Chlamydomonas reinhardtii, but also contains a C-terminal endoplasmic reticulum (ER) retention signal, -KDEL. Here, we show by fluorescence microscopy that RB60 resides in the chloroplast but also outside of the chloroplast colocalized with BiP, an ER marker protein. RB60 accumulates in microsomes that exhibit a typical ER magnesium-shift, and cotranslationally translocates into ER microsomes. The first 50-aa leader of RB60 is sufficient for both chloroplast and ER targeting. The leader is cleaved upon translocation into the ER, whereas it remains intact after import to the chloroplast. The leader sequence also contains an acidic domain that appears necessary for the protein's association with the thylakoid membranes. Based on these and additional results, we propose that the dual localization of RB60 occurs via the two conserved transport mechanisms, to the chloroplast and to the ER, that the chloroplast RB60 most likely carries an additional function in the ER, and that its mode of transport, including the differential cleavage of its N terminus, plays an important role in its suborganellar localization and organellar-specific function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.