ArticleProceedings of the National Academy of Sciences of the United States of America2005
Synaptic vesicle recycling studied in transgenic mice expressing synaptopHluorin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
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Who cites it
91 citing papers in PubMed, 154 citations in OpenAlex.
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- Genetically encoded sensors illuminateIBRO neuroscience reports · 2025Article
- PHluorin-conjugated secondary nanobodies as a tool for measuring synaptic vesicle exocytosis and endocytosis.Scientific reports · 2025Article
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- Synaptotagmin-11 Inhibits Synaptic Vesicle Endocytosis via Endophilin A1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- The SphK1/S1P Axis Regulates Synaptic Vesicle Endocytosis via TRPC5 Channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Methods of measuring presynaptic function with fluorescence probes.Applied microscopy · 2021Review
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- Disturbed neurotransmitter homeostasis in ether lipid deficiency.Human molecular genetics · 2019Article
- Molecular tools for imaging and recording neuronal activity.Nature chemical biology · 2019Review
- Altered Proteins in the Hippocampus of Patients with Mesial Temporal Lobe Epilepsy.Pharmaceuticals (Basel, Switzerland) · 2018Article
- Lighting up the brain: genetically encoded fluorescent sensors for imaging neurotransmitters and neuromodulators.Current opinion in neurobiology · 2018Review
- Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity.eLife · 2018Article
- Novel pH-Sensitive Lipid Based Exo-Endocytosis Tracers Reveal Fast Intermixing of Synaptic Vesicle Pools.Frontiers in cellular neuroscience · 2018Article
- Optogenetic and chemogenetic techniques for neurogastroenterology.Nature reviews. Gastroenterology & hepatology · 2018Review
- Diverse modes of synaptic signaling, regulation, and plasticity distinguish two classes ofeLife · 2017Article
- Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons.Journal of visualized experiments : JoVE · 2017Article
- Comparative ultrastructural features of excitatory synapses in the visual and frontal cortices of the adult mouse and monkey.The Journal of comparative neurology · 2017Article
31 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synaptic vesicles are recycled locally within presynaptic specializations. We examined how vesicles are reused after endocytosis, using transgenic mice expressing the genetically encoded fluorescent indicator synaptopHluorin in subsets of neurons. At both excitatory and inhibitory synapses in cultured hippocampal neurons, newly endocytosed vesicles did not preferentially enter the releasable pool of vesicles. Rather, they entered the reserve pool first and subsequently the readily releasable pool over a period of several minutes. All vesicles in the recycling pool could be accessed by spaced stimuli, arguing against preferential local reuse of the readily releasable vesicles. Interestingly, nearly half the vesicles at excitatory synapses, and a third at inhibitory synapses, could not be recruited for release even by sustained stimuli. We conclude that, at presynaptic terminals in the hippocampus, most vesicles vacate release sites after exocytosis and are replaced by existing vesicles from the reserve pool, placing constraints on kiss-and-run recycling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.