ArticleGenetic vaccines and therapy2005
Electroporation by nucleofector is the best nonviral transfection technique in human endothelial and smooth muscle cells.
Article in Genetic vaccines and therapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Electroporation outcomes in human Jurkat cells are affected by exposure to simulated microgravity.Research square · 2026Article
- Article
- Ex Vivo Delivery of mRNA to Immune Cells via a Nonendosomal Route Obviates the Need for Nucleoside Modification.ACS bio & med chem Au · 2024Article
- Enhancement effect of urea toward electroporation-mediated plasmid transfection efficiency in the HEK-293 cell line.Research in pharmaceutical sciences · 2024Article
- Effect of Honokiol on culture time and survival of Alzheimer's disease iPSC-derived neurons.BioImpacts : BI · 2024Article
- Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.Pharmaceutics · 2022Review
- Novel immortalized human vocal fold epithelial cell line: In vitro tool for mucosal biology.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!Pharmaceutics · 2020Review
- An improved method for increasing the efficiency of gene transfection and transduction.International journal of physiology, pathophysiology and pharmacology · 2018Article
- Intracellular tracking of single-plasmid DNA particles after delivery by electroporation.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Combination of Sleeping Beauty transposition and chemically induced dimerization selection for robust production of engineered cells.Nucleic acids research · 2012Article
- Biochemical modulation of aracytidine (Ara-C) effects by GTI-2040, a ribonucleotide reductase inhibitor, in K562 human leukemia cells.The AAPS journal · 2011Article
- Article
- Assessment of methods and analysis of outcomes for comprehensive optimization of nucleofection.Genetic vaccines and therapy · 2009Article
- Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method.World journal of gastroenterology · 2007Article
- A platform of high-efficiency nonviral gene transfer in mouse osteoblast cells in vitro.Molecular biotechnology · 2006Article
- Comparison of nonviral transfection and adeno-associated viral transduction on cardiomyocytes.Molecular biotechnology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aim of this study was to determine the optimal non-viral transfection method for use in human smooth muscle cells (SMC) and endothelial cells (EC).
methodsCoronary Artery (CoA) and Aortic (Ao) SMC and EC were transfected with a reporter plasmid, encoding chloramphenicol acetyltransferase type 1 (CAT), with seven different transfection reagents, two electroporation methods and a photochemical internalization (PCI) method. CAT determination provided information regarding transfection efficiency and total protein measurement was used to reflect the toxicity of each method.
resultsElectroporation via the nucleofector machine was the most effective method tested. It exhibited a 10 to 20 fold (for SMC and EC, respectively) increase in transfection efficiency in comparison to the lipofection method combined with acceptable toxicity. FuGene 6 and Lipofectamine PLUS were the preferred transfection reagents tested and resulted in 2 to 60 fold higher transfection efficiency in comparison to the PCI which was the least effective method.
conclusionThis study indicates that electroporation via the nucleofector machine is the preferred non-viral method for in vitro transfection of both human aortic and coronary artery SMC and EC. It may be very useful in gene expression studies in the field of vascular biology. Through improved gene transfer, non-viral transfer techniques may also play an increasingly important role in delivering genes to SMC and EC in relevant disease states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.