ArticleJournal of medical genetics1992
Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics.
Article in Journal of medical genetics, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 216 citations in OpenAlex.
- Chromosome Analysis and Sorting Using Conventional Flow Cytometers.Current protocols · 2023Article
- Observations on chromosome-specific sequencing for the construction of cross-species chromosome homology maps and its resolution of human:alpaca homology.Molecular cytogenetics · 2022Review
- Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA.Scientific reports · 2021Article
- Review
- Chromosome Analysis Using Benchtop Flow Analysers and High Speed Cell Sorters.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Programmable Chromosome Painting with Oligopaints.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Homologue-specific chromosome sequencing characterizes translocation junctions and permits allelic assignment.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Chromosomal breaks at FRA18C: association with reducedNPJ precision oncology · 2017Article
- 14q32.3-qter trisomic segment: a case report and literature review.Molecular cytogenetics · 2016Article
- History and evolution of cytogenetics.Molecular cytogenetics · 2015Review
- Human molecular cytogenetics: From cells to nucleotides.Genetics and molecular biology · 2014Review
- Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012Review
- Characterising chromosome rearrangements: recent technical advances in molecular cytogenetics.Heredity · 2012Review
- Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays.Nature protocols · 2009Article
- Flow analysis and sorting of microchromosomes (<3 Mb).Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2007Article
- The molecular basis of chromosome orthologies and sex chromosomal differentiation in palaeognathous birds.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Article
- Micro-array analyses decipher exceptional complex familial chromosomal rearrangement.Human genetics · 2006Article
- Molecular cytogenetic definition of the chicken genome: the first complete avian karyotype.Genetics · 2004Article
- Applications of combined DNA microarray and chromosome sorting technologies.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Article
- A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization.Genome research · 2004Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a method, termed reverse chromosome painting, which allows the rapid analysis of the content and breakpoints of aberrant chromosomes. The method involves the sorting of small numbers of the aberrant chromosome from short term blood culture preparations or cell lines by using bivariate flow karyotype analysis. The sorted chromosomes are amplified and biotin labelled enzymatically using a degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR), the product annealed to metaphase spreads from normal subjects, and hybridisation detected using fluorescence in situ hybridisation (FISH). We show the usefulness of this method for routine clinical cytogenetics by the analysis of cases involving an insertion, a deletion, a translocation, and two cases of a chromosome with additional material of unknown origin. The method has particular application for the rapid resolution of the origin of de novo unbalanced chromosome duplications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.