ArticleThe British journal of ophthalmology2005
Corneal graft rejection occurs despite Fas ligand expression and apoptosis of infiltrating cells.
Article in The British journal of ophthalmology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 14 citations in OpenAlex.
- A Novel Rat Model of Dry Eye Induced by Aerosol Exposure of Particulate Matter.Investigative ophthalmology & visual science · 2022Article
- Air pollutant particulate matter 2.5 induces dry eye syndrome in mice.Scientific reports · 2018Article
- Gene Therapy for Modulation of T-Cell-Mediated Immune Response Provoked by Corneal Transplantation.Human gene therapy · 2018Article
- A murine model of dry eye induced by topical administration of erlotinib eye drops.International journal of molecular medicine · 2018Article
- Concise review: immunological properties of ocular surface and importance of limbal stem cells for transplantation.Stem cells translational medicine · 2013Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND/
aimsConstitutive expression of Fas ligand (CD95L) protects the eye against cell mediated immune responses by inducing apoptosis in infiltrating Fas bearing T cells. This study was designed to examine Fas ligand expression on acutely rejecting rat corneal grafts and to investigate the kinetics of induction of apoptosis in infiltrating leucocytes.
methodsOrthotopic penetrating corneal transplantation was performed between genetically disparate inbred rats. Fas ligand expression and the phenotype of infiltrating leucocytes were examined by immunohistochemistry. Apoptotic nuclei were visualised in sections of normal rat cornea, rejecting allografts, and time matched isografts by terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labelling (TUNEL) and quantified by video image analysis. Staining with Hoechst dye 33258 was used to confirm the presence of apoptotic nuclei.
resultsFas ligand was expressed on corneal endothelial and epithelial cells during acute corneal graft rejection. At all time points examined, including as early as the fifth postoperative day, the cells infiltrating both corneal isografts and allografts were TUNEL positive. By the 15th postoperative day, over 90% of all nuclei, many of which were T cells, were apoptotic.
conclusionExpression of Fas ligand is not downregulated on the cornea during allograft rejection and infiltrating leucocytes in both isografts and allografts die rapidly in situ. Despite the death of the cells believed to be responsible for rejection, isografts survive indefinitely whereas allografts are irreparably damaged.
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