ArticleGenes & development2005
Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed.
- Chromatin architecture sets origin licensing capacity.Research square · 2026Article
- Chromatin architecture sets origin licensing capacity.bioRxiv : the preprint server for biology · 2026Article
- ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.Nature structural & molecular biology · 2024Article
- The choreography of chromatin in RNA polymerase III regulation.Biochemical Society transactions · 2024Review
- ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.bioRxiv : the preprint server for biology · 2023Article
- Article
- In vivo analysis reveals that ATP-hydrolysis couples remodeling to SWI/SNF release from chromatin.eLife · 2021Article
- Article
- Light and shadow on the mechanisms of integration site selection in yeast Ty retrotransposon families.Current genetics · 2021Review
- Article
- Verticillium dahliae chromatin remodeling facilitates the DNA damage repair in response to plant ROS stress.PLoS pathogens · 2020Article
- Heterozygous Mutations in SMARCA2 Reprogram the Enhancer Landscape by Global Retargeting of SMARCA4.Molecular cell · 2019Article
- The ATP-dependent chromatin remodelling enzyme Uls1 prevents Topoisomerase II poisoning.Nucleic acids research · 2019Article
- RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast.Molecular cell · 2019Article
- Chromatin Remodeling Factors Isw2 and Ino80 Regulate Chromatin, Replication, and Copy Number of theGenetics · 2018Article
- Retrotransposon targeting to RNA polymerase III-transcribed genes.Mobile DNA · 2018Review
- Blocking promiscuous activation at cryptic promoters directs cell type-specific gene expression.Science (New York, N.Y.) · 2017Article
- Integration site selection by retroviruses and transposable elements in eukaryotes.Nature reviews. Genetics · 2017Review
- Differential roles of the RNases H in preventing chromosome instability.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Sequence-targeted nucleosome sliding in vivo by a hybrid Chd1 chromatin remodeler.Genome research · 2016Article
17 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Isw2 ATP-dependent chromatin-remodeling activity is targeted to early meiotic and MATa-specific gene promoters in Saccharomyces cerevisiae. Unexpectedly, preferential cross-linking of wild-type Isw2p was not detected at these loci. Instead, the catalytically inactive Isw2p-K215R mutant is enriched at Isw2 targets, suggesting that Isw2p-K215R, but not wild-type Isw2p, is a sensitive chromatin immunoprecipitation (ChIP) reagent for marking sites of Isw2 activity in vivo. Genome-wide ChIP analyses confirmed this conclusion and identified tRNA genes (tDNAs) as a new class of Isw2 targets. Loss of Isw2p disrupted the periodic pattern of Ty1 integration upstream of tDNAs, but did not affect transcription of tDNAs or the associated Ty1 retrotransposons. In addition to identifying new Isw2 targets, our localization studies have important implications for the mechanism of Isw2 association with chromatin in vivo. Target-specific enrichment of Isw2p-K215R, not wild-type Isw2p, suggests that Isw2 is recruited transiently to remodel chromatin structure at these sites. In contrast, we found no evidence for Isw2 function at sites preferentially enriched by wild-type Isw2p, leading to our proposal that wild-type Isw2p cross-linking reveals a scanning mode of the complex as it surveys the genome for its targets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.