ArticleMolecular and cellular biology2005
Small interfering RNAs that trigger posttranscriptional gene silencing are not required for the histone H3 Lys9 methylation necessary for transgenic tandem repeat stabilization in Neurospora crassa.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
- Genetic and Epigenetic Mechanisms Underlying Reversible Adaptive Responses in Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- Copy number-dependent DNA methylation of the Pyricularia oryzae MAGGY retrotransposon is triggered by DNA damage.Communications biology · 2021Article
- H3K9me3 is required for inheritance of small RNAs that target a unique subset of newly evolved genes.eLife · 2019Article
- Partition of Repeat-Induced Point Mutations Reveals Structural Aspects of Homologous DNA-DNA Pairing.Biophysical journal · 2018Article
- Repeat-Induced Point Mutation and Other Genome Defense Mechanisms in Fungi.Microbiology spectrum · 2017Review
- DNA sequence homology induces cytosine-to-thymine mutation by a heterochromatin-related pathway in Neurospora.Nature genetics · 2017Article
- Genome-wide redistribution of H3K27me3 is linked to genotoxic stress and defective growth.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mechanism of siRNA production from repetitive DNA.Genes & development · 2015Article
- Heterochromatin controls γH2A localization in Neurospora crassa.Eukaryotic cell · 2014Article
- Neurospora crassa, a model system for epigenetics research.Cold Spring Harbor perspectives in biology · 2013Review
- RNA interference and heterochromatin assembly.Cold Spring Harbor perspectives in biology · 2011Review
- RNA interference in fungi: pathways, functions, and applications.Eukaryotic cell · 2011Review
- RNA interference pathways in filamentous fungi.Cellular and molecular life sciences : CMLS · 2010Review
- Confining euchromatin/heterochromatin territory: jumonji crosses the line.Genes & development · 2010Review
- In vivo trans-specific gene silencing in fungal cells by in planta expression of a double-stranded RNA.BMC biology · 2010Article
- pHg/pSILBAγ vector system for efficient gene silencing in homobasidiomycetes: optimization of ihpRNA - triggering in the mycorrhizal fungus Laccaria bicolor.Microbial biotechnology · 2010Article
- Control of chicken CR1 retrotransposons is independent of Dicer-mediated RNA interference pathway.BMC biology · 2009Article
- Relics of repeat-induced point mutation direct heterochromatin formation in Neurospora crassa.Genome research · 2009Article
- Quelling targets the rDNA locus and functions in rDNA copy number control.BMC microbiology · 2009Article
- RNA and transcriptional modulation of gene expression.Cell cycle (Georgetown, Tex.) · 2008Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Neurospora crassa, the introduction of a transgene can lead to small interfering RNA (siRNA)-mediated posttranscriptional gene silencing (PTGS) of homologous genes. siRNAs can also guide locus-specific methylation of Lys9 of histone H3 (Lys9H3) in Schizosaccharomyces pombe. Here we tested the hypothesis that transgenically derived siRNAs may contemporaneously both activate the PTGS mechanism and induce chromatin modifications at the transgene and the homologous endogenous gene. We carried out chromatin immunoprecipitation using a previously characterized albino-1 (al-1) silenced strain but detected no alterations in the pattern of histone modifications at the endogenous al-1 locus, suggesting that siRNAs produced from the transgenic locus do not trigger modifications in trans of those histones tested. Instead, we found that the transgenic locus was hypermethylated at Lys9H3 in our silenced strain and remained hypermethylated in the quelling defective mutants (qde), further demonstrating that the PTGS machinery is dispensable for Lys9H3 methylation. However, we found that a mutant in the histone Lys9H3 methyltransferase dim-5 was unable to maintain PTGS, with transgenic copies being rapidly lost, resulting in reversion of the silenced phenotype. These results indicate that the defect in PTGS of the Deltadim-5 strain is due to the inability to maintain the transgene in tandem, suggesting a role for DIM-5 in stabilizing such repeated sequences. We conclude that in Neurospora, siRNAs produced from the transgenic locus are used in the RNA-induced silencing complex-mediated PTGS pathway and do not communicate with an RNAi-induced initiation of transcriptional gene silencing complex to effect chromatin-based silencing.
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