ArticleMolecular and cellular biology2005
Identification in vivo of different rate-limiting steps associated with transcriptional activators in the presence and absence of a GAGA element.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Regulation of Promoter Proximal Pausing of RNA Polymerase II in Metazoans.Journal of molecular biology · 2021Review
- GAGA factor: a multifunctional pioneering chromatin protein.Cellular and molecular life sciences : CMLS · 2021Review
- GAGA factor maintains nucleosome-free regions and has a role in RNA polymerase II recruitment to promoters.PLoS genetics · 2015Article
- Article
- Enforcing the pause: transcription factor Sp3 limits productive elongation by RNA polymerase II.Cell cycle (Georgetown, Tex.) · 2013Article
- Control of transcriptional elongation.Annual review of genetics · 2013Review
- Controlling gene expression in response to stress.Nature reviews. Genetics · 2011Review
- Corepressor-directed preacetylation of histone H3 in promoter chromatin primes rapid transcriptional switching of cell-type-specific genes in yeast.Molecular and cellular biology · 2010Article
- Inducible gene expression: diverse regulatory mechanisms.Nature reviews. Genetics · 2010Review
- Review
- Promoter proximal pausing on genes in metazoans.Chromosoma · 2009Review
- Promoter elements associated with RNA Pol II stalling in the Drosophila embryo.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- NELF and GAGA factor are linked to promoter-proximal pausing at many genes in Drosophila.Molecular and cellular biology · 2008Article
- Transcription regulation through promoter-proximal pausing of RNA polymerase II.Science (New York, N.Y.) · 2008Article
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Authors and funding
3 authors.
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Abstract
We analyzed the impact of a GAGA element on a transgenic promoter in Drosophila melanogaster that was activated by proteins composed of the Tet(on) DNA binding domain and either the heat shock factor (HSF) activation domain or a potent subdomain of VP16. Permanganate footprinting was used to monitor polymerase II (Pol II) on the transgenic promoters in vivo. Activation by Tet(on)-HSF but not by Tet(on)-VP16(A2) required the GAGA element; this correlated with the ability of the GAGA element to establish a paused Pol II. Although the GAGA element was not required for activation by Tet(on)-VP16(A2), the GAGA element greatly accelerated the rate of activation. The permanganate data also provided evidence that Pol II encountered different rate-limiting steps, following initiation in the presence of Tet(on)-HSF and Tet(on)-VP16(A2). The rate-limiting step in the presence of Tet(on)-HSF was release of Pol II paused about 20 to 40 nucleotides downstream from the start site. The rate-limiting step in the presence of Tet(on)-VP16(A2) occurred much closer to the transcription start site. Several biochemical studies have provided evidence for a structural transition shortly after Pol II initiates transcription. The behavior of Pol II in the presence of Tet(on)-VP16(A2) provides the first evidence that this transition occurs in vivo.
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