ArticleMolecular and cellular biology2005
Sequence-specific DNA binding by the alphaNAC coactivator is required for potentiation of c-Jun-dependent transcription of the osteocalcin gene.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 36 citations in OpenAlex.
- NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH.International journal of molecular sciences · 2022Article
- Protein phosphatase 1 regulatory subunit 18 suppresses the transcriptional activity of NFATc1 via regulation of c-fos.Bone reports · 2021Article
- Ubiquitin specific peptidase Usp53 regulates osteoblast versus adipocyte lineage commitment.Scientific reports · 2021Article
- A pipeline to create predictive functional networks: application to the tumor progression of hepatocellular carcinoma.BMC bioinformatics · 2020Article
- Dephosphorylation of the transcriptional cofactor NACA by the PP1A phosphatase enhances cJUN transcriptional activity and osteoblast differentiation.The Journal of biological chemistry · 2019Article
- Regulation of Bone Remodeling by Parathyroid Hormone.Cold Spring Harbor perspectives in medicine · 2018Review
- Parathyroid Hormone Signaling in Osteocytes.JBMR plus · 2018Review
- The PTH-Gαs-protein kinase A cascade controls αNAC localization to regulate bone mass.Molecular and cellular biology · 2014Article
- Article
- Combinatorial control of ATF4-dependent gene transcription in osteoblasts.Annals of the New York Academy of Sciences · 2011Review
- Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.Immunity · 2011Article
- skNAC, a Smyd1-interacting transcription factor, is involved in cardiac development and skeletal muscle growth and regeneration.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Nuclear alpha NAC influences bone matrix mineralization and osteoblast maturation in vivo.Molecular and cellular biology · 2010Article
- FIAT is co-expressed with its dimerization target ATF4 in early osteoblasts, but not in osteocytes.Gene expression patterns : GEP · 2009Article
- Ribosome association and stability of the nascent polypeptide-associated complex is dependent upon its own ubiquitination.Genetics · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since the c-Jun coactivator alphaNAC was initially identified in a differential screen for genes expressed in differentiated osteoblasts, we examined whether the osteocalcin gene, a specific marker of terminal osteoblastic differentiation, could be a natural target for the coactivating function of alphaNAC. We had also previously shown that alphaNAC can specifically bind DNA in vitro, but it remained unclear whether the DNA-binding function of alphaNAC is expressed in vivo or if it is required for coactivation. We have identified an alphaNAC binding site within the murine osteocalcin gene proximal promoter region and demonstrated that recombinant alphaNAC or alphaNAC from ROS17/2.8 nuclear extracts can specifically bind this element. Using transient transfection assays, we have shown that alphaNAC specifically potentiated the c-Jun-dependent transcription of the osteocalcin promoter and that this activity specifically required the DNA-binding domain of alphaNAC. Chromatin immunoprecipitation confirmed that alphaNAC occupies its binding site on the osteocalcin promoter in living osteoblastic cells expressing osteocalcin. Inhibition of the expression of endogenous alphaNAC in osteoblastic cells by use of RNA interference provoked a decrease in osteocalcin gene transcription. Our results show that the osteocalcin gene is a target for the alphaNAC coactivating function, and we propose that alphaNAC is specifically targeted to the osteocalcin promoter through its DNA-binding activity as a means to achieve increased specificity in gene transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.