ArticleProceedings of the National Academy of Sciences of the United States of America2005
Fluorescence measurements reveal stoichiometry of K+ channels formed by modulatory and delayed rectifier alpha-subunits.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Regulation of Kv2.1 Channels by Kv9.1 Variants.Biomedicines · 2025Article
- The Electrically Silent Kv5.1 Subunit Forms Heteromeric Kv2 Channels in Cortical Neurons and Confers Distinct Functional Properties.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- A broad survey of choanoflagellates revises the evolutionary history of the Shaker family of voltage-gated KProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Evaluation of Cell-Free Synthesized Human Channel Proteins for In Vitro Channel Research.Membranes · 2022Article
- Pentameric assembly of the Kv2.1 tetramerization domain.Acta crystallographica. Section D, Structural biology · 2022Article
- Peripheral Voltage-Gated Cation Channels in Neuropathic Pain and Their Potential as Therapeutic Targets.Frontiers in pain research (Lausanne, Switzerland) · 2021Review
- What is the correct stoichiometry of Kv2.1:Kv6.4 heteromers?Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Determining the correct stoichiometry of Kv2.1/Kv6.4 heterotetramers, functional in multiple stoichiometrical configurations.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- KFrontiers in cellular neuroscience · 2020Review
- Conotoxin κM-RIIIJ, a tool targeting asymmetric heteromeric KProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Oddballs in the Shaker family: Kv2-related regulatory subunits.The Journal of general physiology · 2018Article
- The S6 gate in regulatory Kv6 subunits restricts heteromeric KThe Journal of general physiology · 2018Article
- Independent movement of the voltage sensors in KScientific reports · 2017Article
- PIP Water Transport and Its pH Dependence Are Regulated by Tetramer Stoichiometry.Biophysical journal · 2016Article
- Alterations in cortical network oscillations and parvalbumin neurons in schizophrenia.Biological psychiatry · 2015Review
- Silencing of voltage-gated potassium channel KV9.3 inhibits proliferation in human colon and lung carcinoma cells.Oncotarget · 2015Article
- The subfamily-specific interaction between Kv2.1 and Kv6.4 subunits is determined by interactions between the N- and C-termini.PloS one · 2014Article
- Lower gene expression for KCNS3 potassium channel subunit in parvalbumin-containing neurons in the prefrontal cortex in schizophrenia.The American journal of psychiatry · 2014Article
- Functional analysis of missense mutations in Kv8.2 causing cone dystrophy with supernormal rod electroretinogram.The Journal of biological chemistry · 2012Article
- Sensory neuron downregulation of the Kv9.1 potassium channel subunit mediates neuropathic pain following nerve injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Modulatory alpha-subunits, which comprise one-fourth of all voltagegated K(+) channel (Kv) alpha-subunits, do not assemble into homomeric channels, but selectively associate with delayed rectifier Kv2 subunits to form heteromeric channels of unknown stoichiometry. Their distinct expression patterns and unique functional properties have made these channels candidate molecular correlates for a broad set of native K(+) currents. Here, we combine FRET and electrophysiological measurements to determine the stoichiometry and geometry of heteromeric channels composed of the delayed rectifier Kv2.1 subunit and the modulatory Kv9.3 alpha-subunit. Kv channel alpha-subunits were fused with GFP variants, and heteromerization of different combinations of tagged and untagged alpha-subunits was studied. FRET, evaluated by acceptor photobleaching, was only observed upon formation of functional channels. Our results, obtained from two independent experimental paradigms, suggest the formation of heteromeric Kv2.1/Kv9.3 channels of fixed stoichiometry consisting of three Kv2.1 subunits and one Kv9.3 subunit. Strikingly, despite this uneven stoichiometry, we find that heteromeric Kv2.1/Kv9.3 channels maintain a pseudosymmetric arrangement of subunits around the central pore.
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