ArticleGastroenterology2005
Distinct temporal-spatial roles for rho kinase and myosin light chain kinase in epithelial purse-string wound closure.
Article in Gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 123 citations in OpenAlex.
- Reactive oxygen species counteract zebrafish wound contraction and promote wound healing.Biophysical journal · 2025Article
- Molecular mechanism of MLCK1 inducing 5-Fu resistance in colorectal cancer cells through activation of TNFR2/NF-κB pathway.Discover oncology · 2024Article
- Epithelial restitution in 3D - Revealing biomechanical and physiochemical dynamics in intestinal organoids via fs laser nanosurgery.iScience · 2023Article
- Article
- An Overview of Cell Membrane Perforation and Resealing Mechanisms for Localized Drug Delivery.Pharmaceutics · 2022Review
- Review
- Actin-ring segment switching drives nonadhesive gap closure.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Culture of Intestinal Epithelial Cell Monolayers and Their Use in Multiplex Macromolecular Permeability Assays for In Vitro Analysis of Tight Junction Size Selectivity.Current protocols in immunology · 2020Article
- Immune Cell Circuits in Mucosal Wound Healing: Clinical Implications.Visceral medicine · 2020Review
- Contributions of Myosin Light Chain Kinase to Regulation of Epithelial Paracellular Permeability and Mucosal Homeostasis.International journal of molecular sciences · 2020Review
- Tight Junctions as Targets and Effectors of Mucosal Immune Homeostasis.Cellular and molecular gastroenterology and hepatology · 2020Review
- Beneficial effects of nutritional supplements on intestinal epithelial barrier functions in experimental colitis modelsWorld journal of gastroenterology · 2019Review
- Tissue Fluidity Promotes Epithelial Wound Healing.Nature physics · 2019Article
- Changes of tight junction and interleukin-8 expression using a human gastroid monolayer model of Helicobacter pylori infection.Helicobacter · 2019Article
- Forceful closure: cytoskeletal networks in embryonic wound repair.Molecular biology of the cell · 2019Review
- Cathepsin B defines leader cells during the collective invasion of salivary adenoid cystic carcinoma.International journal of oncology · 2019Article
- Graft-versus-host disease propagation depends on increased intestinal epithelial tight junction permeability.The Journal of clinical investigation · 2019Article
- Cell injury triggers actin polymerization to initiate epithelial restitution.Journal of cell science · 2018Article
- The myosin light-chain kinase MLCK-1 relocalizes during Caenorhabditis elegans ovulation to promote actomyosin bundle assembly and drive contraction.Molecular biology of the cell · 2018Article
- Leptin alleviates intestinal mucosal barrier injury and inflammation in obese mice with acute pancreatitis.International journal of obesity (2005) · 2018Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
BACKGROUND &
aimsSmall epithelial wounds heal by purse-string contraction of an actomyosin ring that is regulated by myosin light chain (MLC) kinase (MLCK) and rho kinase (ROCK). These studies aimed to define the roles of these kinases in purse-string wound closure.
methodsOligocellular and single-cell wounds were created in intestinal epithelial monolayers. Fluorescence imaging and electrophysiologic data were collected during wound closure. Human biopsies were studied immunohistochemically.
resultsLive-cell imaging of enhanced green fluorescent protein-beta-actin defined rapid actin ring assembly within 2 minutes after wounding. This progressed to a circumferential ring within 8 minutes that subsequently contracted and closed the wound. We therefore divided this process into 2 phases: ring assembly and wound contraction. Activated rho and ROCK localized to the wound edge during ring assembly. Consistent with a primary role in the assembly phase, ROCK inhibition prevented actin ring assembly and wound closure. ROCK inhibition after ring assembly was complete had no effect. Recruitment and activation of MLCK occurred after ring assembly was complete and coincided with ring contraction. MLCK inhibition slowed and then stopped contraction but did not prevent ring assembly. MLCK inhibition also delayed barrier function recovery. Studies of human colonic biopsy specimens suggest that purse-string wound closure also occurs in vivo, because MLC phosphorylation was enhanced surrounding oligocellular wounds.
conclusionsThese results suggest complementary roles for these kinases in purse-string closure of experimental and in vivo oligocellular epithelial wounds; rho and ROCK are critical for actin ring assembly, while the activity of MLCK drives contraction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.