ArticlePlant physiology2005
A systematic proteomic study of seed filling in soybean. Establishment of high-resolution two-dimensional reference maps, expression profiles, and an interactive proteome database.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
102 citing papers in PubMed.
- Molecular Network Analysis in Model and Non-Model Legumes: Challenges in Omics Data Interpretation Across Species, with a Focus onPlants (Basel, Switzerland) · 2025Review
- Identifying the most sensitive growth stages of soybean to defoliation.Scientific reports · 2025Article
- Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations.BMC plant biology · 2025Article
- Unravelling the Significance of Seed Proteomics: Insights into Seed Development, Function, and Agricultural Applications.The protein journal · 2024Review
- Harnessing Multi-Omics Strategies and Bioinformatics Innovations for Advancing Soybean Improvement: A Comprehensive Review.Plants (Basel, Switzerland) · 2024Review
- Phenol-Based Protein Extraction Method for Plant Proteomic Studies.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The sustainability paradox of processing plant proteins.NPJ science of food · 2023Article
- Multi-omics assisted breeding for biotic stress resistance in soybean.Molecular biology reports · 2023Review
- Comparative transcriptome analysis in peaberry and regular bean coffee to identify bean quality associated genes.BMC genomic data · 2023Article
- Exogenous Spermidine Promotes Germination of Aged Sorghum Seeds by Mediating Sugar Metabolism.Plants (Basel, Switzerland) · 2022Article
- Multi-Omics Techniques for Soybean Molecular Breeding.International journal of molecular sciences · 2022Review
- The role of 14-3-3 proteins in plant growth and response to abiotic stress.Plant cell reports · 2022Review
- Suppression ofFrontiers in plant science · 2022Article
- Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.Frontiers in aging neuroscience · 2022Article
- Enzymatic degradation is an effective means to reduce aflatoxin contamination in maize.BMC biotechnology · 2021Article
- Transcriptome Analysis Reveals Genes of Flooding-Tolerant and Flooding-Sensitive Rapeseeds Differentially Respond to Flooding at the Germination Stage.Plants (Basel, Switzerland) · 2021Article
- Chromatography-Independent Fractionation and Newly Identified Molecular Features of the Adzuki Bean (International journal of molecular sciences · 2021Article
- Changes in the Flower and Leaf Proteome of Common Buckwheat (International journal of molecular sciences · 2021Article
- Metabolite Profiling and Network Analysis Reveal Coordinated Changes in Low-N Tolerant and Low-N Sensitive Maize Genotypes under Nitrogen Deficiency and Restoration Conditions.Plants (Basel, Switzerland) · 2020Article
- Overexpression of ATP sulfurylase improves the sulfur amino acid content, enhances the accumulation of Bowman-Birk protease inhibitor and suppresses the accumulation of the β-subunit of β-conglycinin in soybean seeds.Scientific reports · 2020Article
42 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high-throughput proteomic approach was employed to determine the expression profile and identity of hundreds of proteins during seed filling in soybean (Glycine max) cv Maverick. Soybean seed proteins were analyzed at 2, 3, 4, 5, and 6 weeks after flowering using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This led to the establishment of high-resolution proteome reference maps, expression profiles of 679 spots, and corresponding matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra for each spot. Database searching with these spectra resulted in the identification of 422 proteins representing 216 nonredundant proteins. These proteins were classified into 14 major functional categories. Proteins involved in metabolism, protein destination and storage, metabolite transport, and disease/defense were the most abundant. For each functional category, a composite expression profile is presented to gain insight into legume seed physiology and the general regulation of proteins associated with each functional class. Using this approach, an overall decrease in metabolism-related proteins versus an increase in proteins associated with destination and storage was observed during seed filling. The accumulation of unknown proteins, sucrose transport and cleavage enzymes, cysteine and methionine biosynthesis enzymes, 14-3-3-like proteins, lipoxygenases, storage proteins, and allergenic proteins during seed filling is also discussed. A user-intuitive database (http://oilseedproteomics.missouri.edu) was developed to access these data for soybean and other oilseeds currently being investigated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.