ArticleThe Journal of general physiology2005
AKT2/3 subunits render guard cell K+ channels Ca2+ sensitive.
Article in The Journal of general physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 70 citations in OpenAlex.
- A PIF-SAUR module safeguards hypocotyl elongation from ABA inhibition in the dark.Science advances · 2025Article
- Fusaric acid-mediated S-glutathionylation of MaAKT1 channel confers the virulence of Foc TR4 to banana.PLoS pathogens · 2025Article
- Article
- Structural and Functional Insights into the Role of Guard Cell Ion Channels in Abiotic Stress-Induced Stomatal Closure.Plants (Basel, Switzerland) · 2021Review
- HvAKT2 and HvHAK1 confer drought tolerance in barley through enhanced leaf mesophyll HPlant biotechnology journal · 2020Article
- Functional Characterization of theFrontiers in plant science · 2020Article
- The malate-activated ALMT12 anion channel in the grassThe Journal of biological chemistry · 2019Article
- Diel rewiring and positive selection of ancient plant proteins enabled evolution of CAM photosynthesis in Agave.BMC genomics · 2018Article
- Roles of Chloroplast Retrograde Signals and Ion Transport in Plant Drought Tolerance.International journal of molecular sciences · 2018Review
- The receptor-like pseudokinase MRH1 interacts with the voltage-gated potassium channel AKT2.Scientific reports · 2017Article
- The Arabidopsis AtPP2CA Protein Phosphatase Inhibits the GORK K+ Efflux Channel and Exerts a Dominant Suppressive Effect on Phosphomimetic-activating Mutations.The Journal of biological chemistry · 2016Article
- Arabidopsis CALCIUM-DEPENDENT PROTEIN KINASE8 and CATALASE3 Function in Abscisic Acid-Mediated Signaling and H2O2 Homeostasis in Stomatal Guard Cells under Drought Stress.The Plant cell · 2015Article
- Strategies for improving potassium use efficiency in plants.Molecules and cells · 2014Review
- A Single-Pore Residue Renders the Arabidopsis Root Anion Channel SLAH2 Highly Nitrate Selective.The Plant cell · 2014Article
- Calcium: The Missing Link in Auxin Action.Plants (Basel, Switzerland) · 2013Review
- The role of K(+) channels in uptake and redistribution of potassium in the model plant Arabidopsis thaliana.Frontiers in plant science · 2013Article
- The SNAC1-targeted gene OsSRO1c modulates stomatal closure and oxidative stress tolerance by regulating hydrogen peroxide in rice.Journal of experimental botany · 2013Article
- Article
- Guard cell anion channel SLAC1 is regulated by CDPK protein kinases with distinct Ca2+ affinities.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Preferential KAT1-KAT2 heteromerization determines inward K+ current properties in Arabidopsis guard cells.The Journal of biological chemistry · 2010Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inward-rectifying K+ channels serve as a major pathway for Ca2+-sensitive K+ influx into guard cells. Arabidopsis thaliana guard cell inward-rectifying K+ channels are assembled from multiple K+ channel subunits. Following the recent isolation and characterization of an akt2/3-1 knockout mutant, we examined whether the AKT2/3 subunit carries the Ca2+ sensitivity of the guard cell inward rectifier. Quantification of RT-PCR products showed that despite the absence of AKT2 transcripts in guard cells of the knockout plant, expression levels of the other K+ channel subunits (KAT1, KAT2, AKT1, and AtKC1) remained largely unaffected. Patch-clamp experiments with guard cell protoplasts from wild type and akt2/3-1 mutant, however, revealed pronounced differences in Ca2+ sensitivity of the K+ inward rectifier. Wild-type channels were blocked by extracellular Ca2+ in a concentration- and voltage-dependent manner. Akt2/3-1 mutants lacked the voltage-dependent Ca2+ block, characteristic for the K+ inward rectifier. To confirm the akt2/3-1 phenotype, two independent knockout mutants, akt2-1 and akt2::En-1 were tested, demonstrating that the loss of AKT2/3 indeed affects the Ca2+ dependence of guard cell inward rectifier. In contrast to AKT2 knockout plants, AKT1, AtKC1, and KAT1 loss-of-function mutants retained Ca2+ block of the guard cell inward rectifier. When expressed in HEK293 cells, AKT2 channel displayed a pronounced susceptibility toward extracellular Ca2+, while the dominant guard cell K+ channel KAT2 was Ca2+ insensitive. Thus, we conclude that the AKT2/3 subunit constitutes the Ca2+ sensitivity of the guard cell K+ uptake channel.
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