ArticleThe Journal of cell biology2005
An internal GAP domain negatively regulates presynaptic dynamin in vivo: a two-step model for dynamin function.
Article in The Journal of cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- Review
- Role of Clathrin and Dynamin in Clathrin Mediated Endocytosis/Synaptic Vesicle Recycling and Implications in Neurological Diseases.Frontiers in cellular neuroscience · 2021Review
- Review
- Structural and functional characterization of the dominant negative P-loop lysine mutation in the dynamin superfamily protein Vps1.Protein science : a publication of the Protein Society · 2020Article
- The structural biology of the dynamin-related proteins: New insights into a diverse, multitalented family.Traffic (Copenhagen, Denmark) · 2019Review
- Structural Insights into the Mechanism of Dynamin Superfamily Proteins.Trends in cell biology · 2019Review
- Structure of a mitochondrial fission dynamin in the closed conformation.Nature structural & molecular biology · 2018Article
- Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis.Neurology. Genetics · 2015Article
- Dynamin regulates metaphase furrow formation and plasma membrane compartmentalization in the syncytial Drosophila embryo.Biology open · 2015Article
- Cytoplasmic interaction of the tumour suppressor protein hSNF5 with dynamin-2 controls endocytosis.Oncogene · 2014Article
- Dynamin photoinactivation blocks Clathrin and α-adaptin recruitment and induces bulk membrane retrieval.The Journal of cell biology · 2014Article
- Dynamin rings: not just for fission.Traffic (Copenhagen, Denmark) · 2013Review
- Quantifying the dynamic interactions between a clathrin-coated pit and cargo molecules.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Dynamin-2 function and dysfunction along the secretory pathway.Frontiers in endocrinology · 2013Review
- Article
- Dynamin, a membrane-remodelling GTPase.Nature reviews. Molecular cell biology · 2012Review
- A feedback loop between dynamin and actin recruitment during clathrin-mediated endocytosis.PLoS biology · 2012Article
- The crystal structure of dynamin.Nature · 2011Article
- Drosophila rolling blackout displays lipase domain-dependent and -independent endocytic functions downstream of dynamin.Traffic (Copenhagen, Denmark) · 2010Article
- A role for the dynamin-like protein Vps1 during endocytosis in yeast.Journal of cell science · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The mechanism by which the self-assembling GTPase dynamin functions in vesicle formation remains controversial. Point mutations in shibire, the Drosophila dynamin, cause temperature-sensitive (ts) defects in endocytosis. We show that the ts2 mutation, which occurs in the switch 2 region of dynamin's GTPase domain, compromises GTP binding affinity. Three second-site suppressor mutations, one in the switch 1 region of the GTPase domain and two in the GTPase effector domain (GED), dynamin's putative GAP, fully rescue the shi(ts2) defects in synaptic vesicle recycling. The functional rescue in vivo correlates with a reduction in both the basal and assembly-stimulated GTPase activity in vitro. These findings demonstrate that GED is indeed an internal dynamin GAP and establish that, as for other GTPase superfamily members, dynamin's function in vivo is negatively regulated by its GAP activity. Based on these and other observations, we propose a two-step model for dynamin during vesicle formation in which an early regulatory GTPase-like function precedes late, assembly-dependent steps during which GTP hydrolysis is required for vesicle release.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.