ArticleNucleic acids research2005
Torque-limited RecA polymerization on dsDNA.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- How strand exchange protein function benefits from ATP hydrolysis.Current opinion in genetics & development · 2021Review
- Single-molecule insight into stalled replication fork rescue in Escherichia coli.Nucleic acids research · 2021Review
- Single Molecule Study of the Polymerization of RecA on dsDNA: The Dynamics of Individual Domains.Frontiers in molecular biosciences · 2021Article
- Disturbance-free rapid solution exchange for magnetic tweezers single-molecule studies.Nucleic acids research · 2015Article
- Mediators of homologous DNA pairing.Cold Spring Harbor perspectives in biology · 2014Review
- Article
- Dynamics and Regulation of RecA Polymerization and De-Polymerization on Double-Stranded DNA.PloS one · 2013Article
- RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA replication fork rescue.Nature communications · 2013Article
- RecA homology search is promoted by mechanical stress along the scanned duplex DNA.Nucleic acids research · 2012Article
- Investigating Deinococcus radiodurans RecA protein filament formation on double-stranded DNA by a real-time single-molecule approach.Biochemistry · 2011Article
- Freely orbiting magnetic tweezers to directly monitor changes in the twist of nucleic acids.Nature communications · 2011Article
- Single-molecule studies of the stringency factors and rates governing the polymerization of RecA on double-stranded DNA.Nucleic acids research · 2011Article
- Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments.Nature methods · 2010Article
- Single-molecule imaging brings Rad51 nucleoprotein filaments into focus.Trends in cell biology · 2010Review
- A high-resolution magnetic tweezer for single-molecule measurements.Nucleic acids research · 2009Article
- Dynamics of RecA filaments on single-stranded DNA.Nucleic acids research · 2009Article
- Single molecule studies of homologous recombination.Molecular bioSystems · 2008Review
- Real-time assembly and disassembly of human RAD51 filaments on individual DNA molecules.Nucleic acids research · 2007Article
- Human Rad51 filaments on double- and single-stranded DNA: correlating regular and irregular forms with recombination function.Nucleic acids research · 2005Article
Corrections and comments
- Erratum issuedDekker, Nynke [corrected to Dekker, Nynke H]
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The assembly of RecA onto a torsionally constrained double-stranded DNA molecule was followed in real time using magnetic tweezers. Formation of a RecA-DNA filament on the DNA tether was stalled owing to different physical processes depending on the applied stretching force. For forces up to 3.6 pN, the reaction stalled owing to the formation of positive plectonemes in the remaining DNA molecule. Release of these plectonemes by rotation of the magnets led to full coverage of the DNA molecule by RecA. At stretching forces larger than 3.6 pN, the twist induced during filament formation caused the reaction to stall before positive supercoils were generated. We deduce a maximum built-up torsion of 10.1 +/- 0.7 k(b)T. In vivo this built-up torsion may be used to favor regression of a stalled replication fork or to free the chromosomal DNA in E.coli from its condensing proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.